发酵法生产S-腺苷蛋氨酸前体蛋氨酸补加策略.pdfVIP

生 物 工 程 学 报 Chin J Biotech 2008, October 25; 24(10): 1824-1827 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn © 2008 Institute of Microbiology, CAS CSM, All rights reserved 研究报告 发酵法生产S-腺苷蛋氨酸前体蛋氨酸补加策略 王杰鹏, 谭天伟 北京化工大学生命科学与技术学院, 北京 100029 摘 要: 利用酿酒酵母菌株高密度发酵法生产 S-腺苷蛋氨酸关键的影响因素之一是前体 L-蛋氨酸的补加策略。本研究 采用一支经过常规诱变处理的 S-腺苷蛋氨酸优势积累菌株酿酒酵母 SAM0801, 通过 5 L 发酵罐高密度发酵实验研究, 考察了6 种补加策略, 最终确定了L-蛋氨酸的加入时机为30 h 左右, 当菌体干重达到100 g/L 时, 补加量为每罐40 g L- 蛋氨酸, 发酵 58 h 左右达到最高生物量干重 168 g/L, 产量 14.48 g/L。 关键词: S-腺苷蛋氨酸, 酿酒酵母, 高密度发酵, L-蛋氨酸 Pre-L-methionine Feeding Strategy for S-adenosyl- L-methionine Fermentative Production Jiepeng Wang, and Tianwei Tan College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China Abstract: The yield of S-adenosyl-L-methionine (SAM) on high-cell-density fermentation by saccharomyces cerevisiae is mostly affected by the feeding strategy of pre-L-methionine. The mutant strain SAM0801 that could accumulate more SAM was used in this study. Six high-cell-density fermentation experiments in 5 L fermentor were investigated to get the optimal feeding time and amount of L-methionine. The results showed that when 40 g L-methionine was added in the fermentor after 30 h fermentation, a dry cell weight of 100 g/L was achieved. Under this condition, after 58 h fermentation, both the dry cell weight and the yield of SAM reached the maximum, 168 g/L and 14.48 g/L respectively. Keywords: S-adenosyl-L-methionine, Saccharomyces cerevisiae, high-cell-density fermentation, L-methionine [1] 时间内均没有文献将其超越。近年来, 国内研究机构 S-腺苷蛋氨酸 (SAM)是