三维细胞培养技术的发展及其在干细胞和肿瘤细胞.PDFVIP

网络出版时间：2015-07-29 11:00:43 网络出版地址：/kcms/detail/31.2035.Q1100.003.html 10.11844/cjcb.2015.08.0137 中国细胞生物学学报 Chinese Journal of Cell Biology 2015, 37(8): DOI: 10.11844/cj cb.2015.08.0137 三维细胞培养技术的发展及其在干细胞和 肿瘤细胞中应用 * 赵典典 侯玲玲 张婧思 胡红刚 晏 琼 (北京交通大学理学院生命科学与生物工程研究院, 北京100044) 摘要 三维细胞培养技术是在二维细胞培养和动物实验模型的基础上发展起来的简单、有 效的细胞培养方式。利用三维细胞培养技术可以更好的模拟体内微环境, 为相关研究领域提供新 的手段。目前, 三维细胞培养技术在组织工程、肿瘤学、再生医学和干细胞生物学等研究领域均 有应用。该文就近年来三维细胞培养技术的发展及其在干细胞和肿瘤细胞中的应用进行综述。 关键词 三维细胞培养技术; 干细胞; 肿瘤细胞 The Development of Three-Dimensional Cell Culture Technology and Its Application in Stem Cells and Tumor Cells Zhao Diandian, Hou Lingling*, Zhang Jingsi, Hu Honggang, Yan Qiong (College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China) Abstract Three-dimensional cell culture technique is a kind of simple and effective culture system which is developed on the basis of the two-dimensional cell culture and in vivo animal model. Three-dimensional cell culture can better mimic the in vivo cell environment and provide a new research tool for the relevant fields. At present, three-dimensional cell culture technique is used in many areas, including tissue engineering, tumor treatment, regenerative medicine and stem cell biology, etc. Here, we review recent research progress in three- dimensional cell culture technique and its application in stem cells and tumor cells. Keywords three-d