睡眠剥夺对大鼠一氧化氮和一氧化氮合酶的影响 - 中国临床心理学杂志.pdf

106 Chinese Journal of Clinical Psychology Vol.10 No.2 2002 ,,,,, ,,　, (宝鸡市解放军第三医院精神科,陕西 宝鸡721004) 【】　:(NO)(NOS)。: (Flower Pot), 、、 NO NOS 。:, SD NO NOS , (P0.01～0.05), (P0.05)。, NO NOS 。:NO NOS , , NO 。 【】　;;; :B845.1　　　:A　　　:1005-3611(2002)02-0106-02　　　 The Effects of Sleep Deprivation on Brain Levels of NitricOxide Synthase and NitricOxide in Rat WU Xing-qu, ANG Lai-qi,WAMG Xiao-feng, et al. LanzhouMilitary Command Institute of MentalHealth,The ThirdHospital of PLA, Baoji 721004, China 【Abstract】　Objective:To explore the brain levels of nitric oxide (NO)and nitric oxide synthase(NOS)in rats after sleep de- privation.Methods:NO levels and NOS activity were assayed indifferent brain regions of Sprague-Dawley rats after sleep depri- vation by flower pot comparewith normal group (CC)and tank control groups (TC).Results:NO levels and NOS activity were increased in front cortex and hippocampus after SD compared with CC and TC (P0.01～0.05). onclusion:The NO levels and activity of NOS are increased after sleep deprivation, NOS/NO may play an important role in learning andmemory and modu- lating sleep 【Key words】　Sleep deprivation;Rat;Nitric oxide;Nitric oxide synthase 　　, 1.2　 ,,。 1.2.1 　动物模型的制备　 [1] [4] , 。 ,30cm×30cm×40cm (NO)(CNS) ,6cm , , , 。 1.0cm。 (NOS)NO , ,,。 NO 、 、, NO 。 , 18.0cm, ,, NO , 。 [2, 3] NOS 。 ,18.0～22.0℃。 NO NOS , NO/NOS ,20℃。 。 ,, SD 。 1.2.2　样本采集与处理　5% 1　 (50mg/kg), , 1.1　 500ml 、、Sprague-Dawley ,,、、 30 , 200～250g( ,, ),, 6 :