吲哚胺2,3双加氧酶对肝癌细胞的作用.pdfVIP

中国卫生检验杂志2008年6月第l8卷第6期 Chinese Journal of Heahh Laboratory Fechnology，Jun 2008；Vol l8 No 6 1117 【临床检验】 吲哚胺2，3双加氧酶对肝癌细胞的作用 杨洁，王琦 (山西医科大学附属第二医院消化内科，太原 030001) [摘要] 目的：用IDO以及色氨酸经IDO催化后分解生成的代谢产物(L一犬尿素、邻氨基苯甲酸、喹啉酸)作用于肝癌 细胞，观察肝癌细胞的生长及凋亡的情况。方法：将IDO作用于肝癌细胞，用MTY法测定细胞的活力，并用IDO的抑制 剂1一MT进行干预。观察各个代谢产物对肝癌细胞生长的影响，用流式细胞仪检测细胞周期、细胞凋亡率以及细胞坏死 率的变化。结果：不同浓度的IDO作用于HepG：细胞具有明显的时间和剂量依赖性，IDO浓度在0．5 mmol／L和 5 mmol／L时可以促进HepG：细胞的生长(P0．05)，加入1一MT后IDO的这一作用受到抑制。只有喹啉酸能发挥提高 细胞坏死率和凋亡率，抑制肝癌细胞的作用。结论：IDO表现为促进肝癌细胞生长的作用，1一MT可以抑制这一作用。L 一 犬尿素、邻氨基苯甲酸能够促进HepG：细胞的生长。喹啉酸对HepG 细胞有抑制生长作用。 [关键词] 肝癌细胞；吲哚胺2，3双加氧酶；1一甲基色氨酸；喹啉酸 [中图分类号] R735．7 [文献标识码] B [文章编号] 1004—8685(2008)06—1117—03 Regulatory effects of indoleamine 2，3一dioxygense on HCC Jie，Wang Qi (Department of Gastroenterology，The Second Affiliated Hospital，Shanxi Medical University，Taiyuan 030001 China) [Abstract] Objective：To investigate the change of hepatocyte activation and apoptosis with IDO and Tryptophan calabolism(L — Kynurenine，Anthranilic acid，Quinolinic acid)．Methods：The activity of cells was detected with MTY method after they were treated with different concentration of IDO．Then investigate the change of the activity after adding 1～MT at the same time．The activity，apoptosis and necrosis were detected with MTY method and flow cytometry assay after influence of different Tryptophan calabolism．Results：The effect of IDO on HepG2 cells was dependent on the time and concentration obviously．At the density of 0．5 mmol／L and 5 mmol／L，IDO can accelerate the growth of HepG2 cell(P0．05)．The effect of IDO was inhibited by I— MT．Among Tryptophan calabolism，only Quinolinic acid can inhibit the growth of HepG2 cell by enhancing apoptosis and necrosis．Conclusion