HPLC法测定人血浆中替米沙坦的浓度 - 迪马科技.PDF

CHINESE JOURNAL OF CLINICAL PHARMACY 2006 15 4 ∀ 245 ∀ HPLC法测定人血浆中替米沙坦的浓度 温预关, 伍苡沁, 刘玉平, 马 崔 ( 广州市脑科医院国家药品临床研究基地, 广州510370) 目的 HPLC 方 DiamonsilTM C18( 250 mm! 4 6 mm , 5 m) , - 0 0 3 mol∀L- 1( 45#55, V/ V) , 0 8 mL∀m in- 1, 300 nm, 380 nm, 结果 3 97 50 % 102 4 1% 94 68% , RSD ∃ 4 89% ( n= 5) RSD ∃ 7 18% ( n= 3) ; 1~ 500 g∀L- 1, : = 10 25A - 0 30 , r = 0 999 9( n= 8) ; 0 5 g∀L- 1( RS, N %3) 结论 ; ; R972 4; R927 2 B 100744 06( 2006) Determination of the contents of telmisartan in blood plasma by HPLC Wen Yuguan , Wu Yiqin , Liu Yuping, Ma Cui ( Clinical Pharmacology Base, Guangzhou BrainHospital, Guangzhou 510370, China) ABSTRACT AIM To develop a HPLC method for quantitat ive determination of telmisartan in blood plasma METH DS T elmisartan w as extracted with ethyl acetate The residues w ere analyzed with a reverse ph ase HPLC system TM - 1 ( Diamonsil C column , 250 mm ! 4 6 mm, 5 m) ; mobile phase w as acetonitrile- 0 03 mol∀L NH AC( 45#55, 18 4 V/ V) ; the fluorimetric excitation and emission w avelengths w ere set at 300 nm and 380 nm, respectively RESULTS The average recoveries for telmisartan w ere 97 50% , 102 41% and 94 68% , respectively The w ithinday( n= 5) and betweenday( n= 3) RSD w as 4 89% and 7 18% , respect ively The calibration curve for telmisartan was linear in the - 1 - 1 range of 1 to 500 g ∀L The limits of quantitation for telmisarta