扛板归中总黄酮和槲皮素的含量测定_蔡小玲.pdfVIP

辽宁中医杂志 2010年第 37卷第 3期 # 511# 1 2 , ( 11 桂林医学院附属医院药剂科, 广西 桂林 541001; 21 桂林医学院, 广西 桂林 541004) : 目的:建立扛板归中总黄酮和槲皮素的含量测定方法方法: 采用紫外- 可见分光光度法测定药 中总黄 - 1 酮含量; RP- HPLC测定药 中槲皮素含量结果:分光光度法测定总黄酮时, 在 01 00864 ~ 0106912mg# mL 的浓度范 围内线性关系良好 ( r 01 9998), 平均 回收率 981 6% RSD 1168% ; RP - HPLC 测定槲皮素时, 在 01008 ~ - 1 01 04m g# mL 的浓度范围内线性关系良好(r 019998) , 平均回收率为 9815% , RSD 11 31% 结论: 所用方法准确简 便, 可用于扛板归药 的质量控制 : 扛板归; 总黄酮; 槲皮素; 含量测定 : R965 : A : 1000- 1719( 2010) 03- 0511- 02 Determ ination Contents of Total flavonoids and Quercetin of Polugomum Perfoliatum L. 1 2 CA I X iao-ling , ZHANG K e-f eng ( 11App iliated H osp ital of Gu ilin M edical University, Guilin 541001, Guang ,i China; 21Guilin M ed ical University, Guilin 541004, Guang i, China) Abstract: Obj ective: To estab lish new m ethods to determ inate the total flavonoids and quercetin of thePolugomum p erf oliatum L.M ethod s: Total flavonoidsw ere determ ined by UV - V is spectrophotom etry and quercetin by RP- HPLC. Results: Indicated the standard curve of total flavonoidsw as linear in the range of 0100864~ 01 06912m gomL- 1 ( r 01 9998) and its average recovery ratio was 9816% ; The standard curve of the quercetinw as linear in the range of 01 008~ 0104m gomL- 1 ( r 01 9998) and the av- erage recovery ratio was 9815% , RSD 1131% . Conclus ion: them ethod is smi p le and reliab le, can be used to control the quality ofP olugom um p erf oliatum L. K ey words:polugomum p erf oliatum L. ; t