三萜醇酮对高同型半胱氨酸血症致兔动脉粥样硬化的影响.pdfVIP

8 · 737· 三萜醇酮对高同型半胱氨酸血症致兔动脉粥样硬化的影响 张晓飞 张力华 薛存宽 何学斌 (华中科技大学同济医学院附属梨园医院老年病科，湖北 武汉 430077) [摘 要) 目的 探讨三萜醇酮类化合物对高同型半胱氨酸血症(HHey)所致兔动脉粥样硬化(As)的影响。方法 用皮下注射DL一蛋氨酸法 建立动物模型，三萜醇酮大、小剂量组同时灌胃给药。实验前、后分别取血检测血浆总同型半胱氨酸(tHey)。12 W后处死动物，检测血脂、超敏c一反 应蛋白(hs—CRP)。用免疫组织化学方法检测主动脉组织内核因子一KB(NF—KB)的表达。结果 三萜醇酮大、小剂量组兔they、血脂、hs—CRP、NF—KB 光密度值明显低于模型组(PO．05)；大剂量三萜醇酮优于小剂量(PO．05)。结论 三萜醇酮可以抑制HHcy诱导的As的发生发展，其作用可能 是通过降低they、抑制NF—KB的激活和降低hs—CRP的生成而实现。 [关键词) 三萜醇酮化合物；高同型半胱氨酸血症；动脉粥样硬化；核因子一KB；超敏c坂应蛋白 [中图分类号] 11259；R285。5 [文献标识码] A [文章编号] 1005-9202l2008)08-0737-03 Effects of triterpenes on hyperhomocysteinemia-induced atherosclerosis in rabbits ZHANG Xiao-Fei，ZHANG Li-Hua，XUE Cun-Kuan，et口L Department of Geriatrics，Liyuan Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430077，Hubei，China 【Abstract】 0bjective To investigate the effects of triterpenes on hyperhomocysteinemia—induced atherosclerosis in rabbits．Methods 28 rabbits(male)were randomly divided into：control，high，low dose，triterpenes and homocysteine model groups．By hypodermic injection of DL—Met，the hyperhomocysteinemia model was made．At the same time，tfiterpenes low and high dose groups were given tfiterpenes through stomach perfusing respectively 25 and 100 mg‘kg～·d一．111 plasma total homocysteine(tHcy)level Was detected before and after experi— menL All the rabbits were killed after 12 weeks and serum fat and high sensitive C reactive protein(hs—CRP)were exam— ined．Immunohistochemical staining Was performed to detect the expression of nuclear factor KB(NF—KB)in the arteriae aorta．Results The tHcy，serum fat，hs—CRP and the expression of NF—KB in triterpenes low and high groups were all lower than those of model group significantly (P0．05)．The triterpenes high dose group Was superior to triterpenes low dose group(P0．05)．Conclusions Triterpenes Can inhibit hy— perhomocyst