三苯氧胺联合人参皂苷Rg3抑制乳腺癌血管生成的研究.pdfVIP

肿瘤2008年4月第28卷第4期 Tumor Vo1．28，No．4，April，2008 ·279· DOI：10．3781／j．issn．1000-7431．2008．04．002 确Is Research。· 基础碍究 三苯氧胺联合人参皂苷Rg3抑制乳腺癌血管生成的研究 康欣梅。，张清媛 ，王恕怀 ，赵文辉 (哈尔滨医科大学附属肿瘤医院1．内科，2．病理科，哈尔滨 150081) [摘要] 目的：研究三苯氧胺(tamoxifen，TAM)联合人参皂苷R 对乳腺癌肿瘤血管生成的影响，并观察其抑瘤效果。方 法：以荷MCF-7乳腺癌裸鼠为模型，分别给予TAM、雌二醇(estradiol，E2)、人参皂苷Rg3、TAM联合人参皂苷Rg3及9％氯化 钠溶液灌胃，观察肿瘤生长情况，免疫组织化学染色计数肿瘤微血管密度(microvascular density，MVD)，PT-PCR法检测血管内 皮生长因子(vascular endothelial growth factor，VEGF)表达。结果：E2处理组的肿瘤生长迅速，而TAM治疗组肿瘤生长受到 抑制，合用人参组皂苷Rg3时抑瘤效果更显著(P0．05)。与9％氯化钠溶液对照组相比，E2组MVD升高，而TAM组和人 参皂苷Rg3组的MVD及VEGF mRNA表达均下降，二者合用时MVD和VEGF mRNA表达下降更明显(P0．05)。结论： TAM可抑制乳腺癌肿瘤血管生成，与人参皂苷R 联合应用显示出抗血管生成协同作用，可有效抑制乳腺癌肿瘤生长。 [关键词l 乳腺肿瘤；三苯氧胺；人参皂苷类；血管内皮生长因子类 [中图分类号] R730．53 [文献标志码] A [文章编号] 1000-7431(2008)04-0279-03 Antiangiogenic effect of tamoxifen combined with nsenoside Rg3 on breast carcinoma KANG Xin-me／ ，ZHANG Qing-yuan ，WANG Shu—huai ，ZHAO Wen-hui (1．Department of Medical Oncology，2．Depart- ment of Pathology，Cancer Hospital，Harbin Medical University，Harbin 15008 l，China) [ABSTRACT] Objective：To evaluate the anti-angiogenic and anti-tumor effects of tamoxifen(TAM)combined with ginsenoside Rg3 on breast carcinoma cell line MCF-7．Methods：Nude mice bearing MCF-7 breast carcinoma were randomized into five groups．The nude mice were administered by TAM，estradiol(E2)，ginsenoside Rg3，TAM combined with ginsenoside ng3，and sterile saline， respectively．Tumor growth was monitored in each group．After three weeks of treatment，tumor microvascular density(MVD)was detected by immunohistochemical staining．The mRNA expression of vascular endothelial growth factor(VEGF)was detected by RT- PCR．Results：Th e MCF-7 breast carcinoma grew rapidly in E2 treatment group．The tumor growth was inhibited in TAM treatment group and the inhibitory ratio was significantly higher in TAM plus ng3 treatment group(P0．05)．Compared with saline control，the MVD and t