实验性变应性鼻炎与血清元素锌铜钙及细胞因子的相关.docVIP

　　实验性变应性鼻炎与血清元素锌铜钙及细胞因子的相关 【摘要】 　　目的通过建立肺气虚变应性鼻炎(AR)病证复合动物模型，分别检测动物体内血清元素及细胞因子水平，观察细胞因子网络Th1/Th2失衡对于AR及肺气虚证的影响，及血清元素在体内的含量变化与细胞因子的相关性。方法采用烟熏法及TDI鼻腔激发法，建立豚鼠肺气虚变应性鼻炎病证结合模型，运用原子吸收分光光度法检测致敏豚鼠血清Zn，Cu，Ca含量，运用双抗体夹心ELISA法检测致敏豚鼠血清IFN-r，IL-4水平，并对元素值和细胞因子水平之间进行相关分析。结果肺气虚变应性鼻炎豚鼠血清IL-4升高，IFN-r下降，IFN-r/IL-4比值降低，与正常对照组比较，差异显著（Plt;0.01），并见血清Zn值升高（Plt;0.05）。相关分析示Zn与IL-4呈正相关，与IFN-r/IL-4比值呈负相关，Ca与IL-4呈负相关，与IFN-r/IL-4比值呈正相关。结论AR疾病及肺气虚证均与Th1/Th2失衡有关，认为变应性鼻炎（AR）是以鼻腔黏膜Th2免疫反应为主的变应性炎症反应，肺气虚证是细胞因子网络自稳态动态平衡破坏的结果；在AR疾病中，Zn，Ca元素参与影响Th1/Th2平衡调节机制，提示可通过血清元素的补损，达到治疗AR的目的。 【关键词】 变应性鼻炎 微量元素 肺气虚证 动物模型 细胞因子 　　Abstract:ObjectiveAnimal model levels of trace elements and cytokines ined in order to investigate the influence of imbalanced Th1/Th2 cytokine ental allergic rhinitis (EAR) in the pattern of LQD and the association of their contents and levels changes ent of this lesion.MethodsIn the experimental studies, the animal model oke inhalation and TDI nose dropping. Then, AAS contents of trace elements Zn, Cu and Ca and double antibody sandeasure the serum levels of IFNγ and IL4 in them. At last, a correlation analysis of trace element contents plications in the pathogenesis of PAR.ResultsAs shoental studies, the modeled animals level of IL4, reduced serum level of IFNγ and declined ratio of IFNγ/ IL4, of the modeled animals (Plt;0.05) as al modeling procedure odel in guinea pigs in a balanced cytokine mation in nasal mucosa of cases ight be mainly induced by dominant Th2 immunoreaction, estatic m echanism of cytokine ic balance.3. In the pathogenesis process of PAR, trace elements Zn and Ca might play some roles in the regulating mechanism of Th1/Th2 cytokine ight be achieved on the treatment of PAR through supplementing or reducing the serum contents of trace element. 　　Key ent; Lung-Qi deficiency pattern; Animal model; Cytokin 　　变应性鼻炎（ Allergic Rhinitis, AR）为耳鼻喉科临床常见病、多发病，本病中医名“鼻鼽”，其病机是肺、脾、肾功能失调，气阳虚衰，临床辨证分型以虚证为主，特别是肺气虚多见。为了进一步探讨本病 “肺气虚证”的实质以及与该病病理过程的相关性，本研究通过检测“变应性鼻炎－肺气虚证”病证复合动物模型血清元素及细