低质量浓度T-2处理对马铃薯块茎苯丙烷代谢活性的诱导.pdfVIP

232 2015,Voi．36,No．06 畏品科学 ※包装贮运 低质量浓度T．2处理对马铃薯块茎 苯丙烷代谢活性的诱导 赵莹，薛华丽，毕阳木，唐亚梅，沈科萍 (甘肃农业大学食品科学与工程学院，甘肃兰州 730070) 摘要：由镰刀菌引起的干腐病是导致甘肃省马铃薯块茎贮藏期间的主要病害。以‘陇薯3号’马铃薯为试材，用 l pg／mLT-2处理马铃薯块茎切片，观察1-2处理对马铃薯块茎切片损伤接种硫色镰刀菌(Fusarium sulphureum)后， 其对病斑直径的抑制效果，以及块茎切片苯丙烷代谢关键酶活性及产物积累的影响。结果表明，T-2处理有效降低 了块茎切片损伤接种Fusarium sulphureum的病斑直径，提高了切片苯丙氨酸解氨酶和4．香豆酰．辅酶A连接酶的活 性，促进了木质素、总酚、类黄酮及花青素的积累。由此表明，低质量浓度1-2处理可通过诱导马铃薯的苯丙烷代 谢增强块茎对干腐病的抗性。 关键词：马铃薯：1-2毒素；诱导抗性 T-2ToxinatLowConcentrationActivates inPotatoTubers PhenylpropanoidPathway ZHAO Huali，BI Yamei，SHEN Ying，XUE Yang木，TANG Keping ofFoodScienceand 730070，China) (College Engineering，GansuAgriculturalUniversity，Lanzhou oneofthemost diseasesof tubers Abstract：Dryrot,causedbyFusarium$ulphureumspp．，is important postharvest potato inGansu tuber Shu treatedwithT-2 at1 ofT．2toxin province．Potatoslices(CV．LongNo．3)were toxinpg／mL．Theefficacy treatmentonthelesiondiameter tuberslicesinoculatedwithE activities involved ofpotato sulphu地oum．theofkeyenzymes in andmetaboliteswere resultsshowedthat1-2toxintreatment phenylpropanoidpathway investigated．111e significantly decreasedlesiondiameter tuberslicesinoculatedwithE enhancedtheactivities ofpotato sulphureum，and ofphenylalanine cournaric A also theaccumulationof ammonia·lyase(PAL)a