β-葡萄糖苷酶基因克隆及在丝状真菌中高效表达的研究进展.pdf

5 10 Vol. 5 No. 10 2014 10 Journal of Food Safety and Quality Oct. , 2014 β- 朱凤妹, 彭丽莎, 李 军* ( , 066600) 摘 要: , (β-l,4)(β-1,4)β-β- , , , , , β-β- β-β- 关键词: β-; ; ; Research progress on β-glucosidase gene cloning and high level expression in filamentous fungi * ZHU Feng-Mei, PENG Li-Sha, LI Jun (College of Food Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066600, China) ABSTRACT: Cellulase is a group of several enzymes produced chiefly by fungi, bacteria, and protozoans which can catalyze cellulolysis, the decomposition of cellulose. It includes endo-1,4-beta-D-glucanase (beta-1, 4-glucanase, beta-1,4-endoglucan hydrolase, endoglucanase D, 1,4-(1,3,1,4)-beta-D-glucan 4-glucanohydrolase) and β-glucosidase. β-glucosidase hydrolyzed cellobiose into 2 molecules of glucose which is the rate-limiting enzyme of cellulase. Moreover, β-glucosidase creates a bottleneck in cellulase hydrolysis owing to low content and poor enzyme activity. It is important to study the high level expression of β-glucosidase gene by construct- ing of engineering bacteria using gene recombination technology. Herein, we review the current progress of studies on the strain screen