小儿急性白血病患者血清VEGF水平和外周血B细胞及T淋巴细胞亚群水平检测的临床意义.pdfVIP

《淮海医药》2008年1月 第26卷 第1期 J Huaihai Med，January 2O08’ o1．26 ． · 7· 小儿急性白血病患者血清VEGF水平 和外周血B细胞及T淋巴细胞亚群水平检测的临床意义 徐丽华 【摘 要】 目的 探讨小儿急性白血病患者血清血管内皮生长因子(VEGF)水平和外周血B和T淋巴细胞亚群水平 及临床意义。方法 应用酶联免疫法(ELISA)和单克隆抗体法对31例小儿急性白血病患者进行了血清VEGF和外 周B和T淋巴细胞亚群水平的检测，并与30名正常健康儿童作比较。结果 小儿急性白血病患者血清VEGF、B淋巴 细胞数均显著地高于正常人组(P0．01)，而CD3、CIM、CD4／CD8比值则显著地低于正常人组(P0．01)。结论 检 测血清VEGF和外周血B细胞和T淋巴细胞亚群水平的变化对观察病情和预后均有十分重要的临床价值。 【关键词】 急性白血病；血管内皮生长因子；B淋巴细胞；T淋巴细胞亚群 【中图分类号】 R733．71 【文献标识码】A 【文章编号】 1008—7044(2008)01-0007-02 Cfi~cal significance of determination of changes of serRm VEGF levels。peripheral B lymphocyte count and T lyre— phocyte subset distribution pattern in patients with children acute leukemia． XU Li—Hua．f Lianyungang First Peoples Ho~ital，Jiangsu 222002，China) 【Abstract】 0bjective To explore the changes of serum VEGF levels。peripheral B cell count and T subsets distribu． tion pattern in patients with children acute leukemia．Methods Serum VEGF levels(with ELISA)，peripheral B cells as well as T subsets(with mono elonal—antibody technique)were examined in 3 1 patients with children acute leukemia and were COB— pared with 30 controls．Results The sertlm VEGF levels and B lymphocytes count were significantly higher than those in con— trols(P0．01)，while the CD3，CD4 and CD4／CD8 ratios were significantly lower than those in controls(P0．01)．Conclu． sions Detection of serum VEGF，B lymphocyte count and T lymphocytes subset distribution pattern levels are closely related to the disease process of children acute leukemia and ware of prognostic value． 【Key words】 Acute leukemia；Vascular endothelial growth factor(VEGF)；B—lymphocytes；T—lymphocytes subset 文献证实…：血管生成受血管生成促进因子和 血管内皮生长因子(VEGF)的作用最强。国内尚少 血管生成抑制因