大豆疫霉菌生物学性状的遗传与变异.pdfVIP

第26卷 第6期 大 豆 科 学 VoL 26 No,6 2007钷 12月 SOYBEAN SCIENCE Dec． 2007 大豆疫霉菌生物学性状的遗传与变异 侯巨梅 ，刘 铜 ，左豫虎。 (1．景德镇高等专科学校生物与化学工程系，景德镇 333000；2．上海交通大学农学院，上海201101；3．黑龙 江八一农垦大学植物科技学院，大庆 163319) 摘要 大豆疫霉菌引起的大豆疫病是大豆上危害严重的毁灭性病害之一。采用经典遗传学方法研 究大豆疫霉茵单孢后代的茵丝生长速率、茵落形态、产孢量以及同宗配合性状的遗传变异，以期为 有效控制大豆疫病的发展和蔓延奠定基础。结果表明，茵落形态、生长速率和同宗配合性状在单孢 后代可稳定遗传，控制上述性状的遗传因子是纯合的；大豆疫霉菌的游动孢子产生能力在单孢后代 发生连续性变异，表明这种性状可能是数量遗传性状，也可能控制这种性状的基因为杂合基因或细 胞质遗传因子。研究结果初步揭示了大豆疫霉的遗传特征、遗传多样性及多样性产生的遗传学 基础。 关键词 大豆疫霉菌；茵落形态；产孢能力；同宗配合性状；遗传变异 中图分类号 $435．651 文献标识码 A 文章编号 1000—9841(2007)O6一O918一O4 INHERITANCE AND VARIATIoN oF BIoLoGICAL CHARACTERISTICS oF P日y DP日TH0RA S0l，AE HOU J u—mei ，LIU Tong。，ZUO Yu—hu。 (1．Department of Biology and Chemical Engineering，J ingdezhen Comprehensive College；J ingdezhen 333000；2．College of Agriculture，Shanghai Jiaotong University；Shanghai 201101；3．College of Plant Science and Technology，HeilongJiang August First Land Reclamation University，Daqing 163319) Abstract Phytophthora sojae causes a major disease of soybean phytophthora root rot．This pa— per researched the inheritance and variation of growth rate，colony morphology，sporulation and homothallism of Phytophthora sojae with classic genetics method．The colony morphology， growth rate and homothallic characters were all steadily inherited in both single-zoospore and sin- gle—oospore progenies，which indicated that the genetic factors of controlling the three characters were homozygous．However，sporulation of zoospore of P．sojae showed successive variation in both single—zoospore and single—oospore progenies，which suggested that the character was con— trolled by heterozygous genotype，or cytoplasmic genetic factor．The results reveal the genetic ba— sis of the inheritance characteristics and diversities of Ph