蛋白质芯片技术介绍.pptVIP

——The future is in your hands! Nowadays,the poteomics(蛋白质的)research is one of a general direction of the development of life science after genomics（基因组学的）research. Protein structure and function have the direct impact on the change of life activity.And the level of gene transcription(转录) can only reflects the change of gene expression products in a certain extent. However, truly functional protein processes after the transcription, translation regulation processing ,controling to form and so on steps. Thus the direct study of protein to the interpretation(翻译) of the real life phenomenonis of great improtance. But research on means and methods(手段方法) of the protein doesnt have a big development, so the search for effective and fast protein analysis technology has become one of the most important step. Then comes the protein chip The protein chip（蛋白芯片） technology is quickly developed in recent years in chemical biology as a high and new technology.It is the basic principle is make various proteins orderly fixed on all kinds of medium carrier（介质载体） as a test chip.And then, using the tag antibodies(抗体) that are specified by the fluorescent(荧光) substance match with the protein.And the corresponding(对应的)protein will matchwith the chip. Then,the fluorescent on the antibody will indicate the corresponding(对应的) number of protein and its expression. All the other antibodies that doesnt make complementary combination will be washed after using the fluorescent scanner(荧光扫描仪)or laser scanning technology(激光扫描共聚技术).Through analysisthe fluorescence intensity(强度)of each point on the chip and interaction relationship between protein and protein, the purpose of achieving determining all sorts of function of gene expression will be done. There are three types of protein microarrays that are currently used to study the biochemical activities of proteins. Analytical microarrays are also known as capture arrays. In this technique, a l