HPLC测定棉籽粕中游离棉酚含量的前处理方法研究.pdfVIP

47 2012 年第37 卷第 12 期 中国油脂 HPLC 定棉籽柏中游离 的 酣含 前处理方法研究 舒静，肖佳妮，张维农 (武汉工业学院食品科学与工程学院，武汉 43∞123) 摘要:研究了高效液相色谱法(HPLC) 测定棉籽柏中游离棉盼含量的前处理方法。利用 70% 丙嗣 水溶液超声辅助提取棉籽柏中的游离棉盼，采用 HPLC 测定其含量，系统考察了提取条件对蒋离棉 盼含量测定的影响。优化后的前处理条件为:液料比26:1 ，超声 60 min ，提取 1 次。在此，条件下， 测定结果稳定性佳(RSD ==2.06%) ，重复性好(RSD == l. 62 % ) ，加标回收率在 97.89% -101. 179岛 之间。该方法可用于棉籽柏中游离棉盼含量的测定。 关键词:棉籽柏:潺离棉盼:前处理方法:高效液相色谱法 中图分类号:TS229;S816 文献标志码:A 文章编号: 1003 -7969 (2012 )12 -∞47 -04 Pretreatment method for detecting free gossypol content in cottonseed meal by HPLC SHU Jing , XIAO Jiani , ZHANG Weinong (College of Food Science and Engineeri吨，Wuhan Polytechnic University ,Wuhan 430023 ,China) Abstract :Pretreatment method for detecting free gossypol content in cottonseed meal by high perfonnance lìquid chromatography( HPLC) was studied. Free gossypol was extracted from cottonseed meal assisted wíth ultrasonic using 70% acetone as solvent. The effects of extraction conditions on determination of free gossypol content were systematically ìnvestìgated by HPLC. The optimal pretreatment conditions were as foUows: ratio of solvent to solid 26 : 1 , ultrasonic time 60 min , extraction once. Under the optimal pre- treatment conditions , the detennination result had virtues of good stability (RSD = 2.06%) and repe卧 ability (RSD = 1. 6213毛)， and the recovery rate was in the range of 97. 891毛- 101. 171毛. This method could be used for determinatÎon of free gossypol content i