BK、SK通道参与慢性损伤坐骨神经异常自发放电节律的调控.pdfVIP

第 42 卷第 1 期 陕西师范大学学报(自然科学版) Vo l. 42 No.1 2014 年 1 月 Journal of Shaanxi Normal University CNatural Science Edition) Jan. , 2014 文章编号: 1672-4291 (2014) 01-0070-06 BK、 SK 通道参与慢性损伤坐骨神经 异常自发放电节律的调控 路美玲，李建宁，魏春玲，刘一辉\任 维 (陕西师范大学生命科学学院，陕西西安 710062) 摘 要:通过运用相对特异的钙激活何通道阻断剂竭毒和峰毒明肤分别阻断 BK 和 SK 通道，观察 对损伤神经自发放电模式的影响，并将其与非特异性匆通道阻断剂四乙基绥的作用进行比较，以揭 示参与调节放电节律的钙激活匆离子通道的亚型.结果显示，竭毒和蜂毒明肤均可以使放电节律主 观分岔转迁.这些结果提示在坐骨神经的损伤区表达有 BK 与 SK 通道，并可能参与调控损伤区神 经纤维的自发放电. 关键词:慢性结扎损伤模型;自发放电;钙激活何通道 中图分类号: Q423 文献标志码:A Calcium-activated potassium channels participate in modifying ectopic discharges of inj ured nerves in CCI model rat LU Meiling , LI Jianning , WEI Chunling , LIU Yihui 椅， REN Wei (College of Life Science , Shaanxi Normal University , Xian 710062 , Shaanxi , China) Abstract: Previous studies on ectopic firing of injured nerves in the chronic constriction injury (CCD model indicated that calcium-activated potassium channels play a key role , but the direct pharmacological evidence has not been reported. This study examined the effect of TEA and rela- tively specific calcium-activated potassium channel blockers IBTX and Apamin on spontaneous fir ing of injury nerves in CCI model rats. The results showed that both IBTX and Apamin could lead to bifurcation scenarios , indicating that the BK and SK channels are involved in the regula- tion of spontaneous firing of injured nerves. The results imply that calcium-activated potassium channels may exist in injured nerves in CCI model , and may be involved in regulation of spontane- ous firing of injured nerves. Key words: chronic constriction injury; ectopic firing; calcium-activated