3种方法测定黑麦种子活力的比较研究.pdfVIP

安徽农业科学，Journal of Anhui A伊. Sci. 2012 ,40(25) : 12410 -12411 , 12433 责任编辑姜丽责任枝对卢瑶 3 种方法测定黑麦种子活力的比较研究 邓娜娜，杜康兮，周志鹏，李立芹* (四川农业大学农学院，四川成都 611130) 摘要 [目的]比较3 种测定黑麦(Secale cereal L. )种子活力的方法，以确定适宜的测定黑麦种子活力的方法。[方法]以黑麦种子为研 究材料，分别用白℃法、红墨水法、紫外吸收分光光皮法3 种常用的方法测定其种子活力，以蒸饱水法为对照。[结果]凹℃法测定的黑 麦种子平均发芽率与对照间不存在显著差异。红墨水法测定的黑麦种子平均发芽率比对照高出了10.13% ，两者之间存在极显著差异。 应用紫外吸收分光光度法测定时，在波长 260 nm 下，浸泡 8 h 后的黑麦种子浸提液的吸光值与种子活力之间有较好的相关性，能够在较 大程度上反映黑麦种子的活力。[结论]该研究可为黑麦种子的生产、贮藏和检验提供理论依据。 关键词 黑麦;紫外吸收分光光度法;π℃法;红墨水法;种子活力 中图分类号 5512.5 变献标识码 A 文章编号 0517 -6611 (2012)25 -12410-但 Comparative Study 00 Thr四 Methods for Determining Rye( Seca/e cereal L. ) seed Vigor DENG Na-na et al (5ichuan Agricultural University , College of Agriculture , Cheng巾， 5ichuan 611130) Abstract [ Objective] The purpo回 was to compare three methods for determing rye seed 吨。r ， and confirm to its su阳ble determination me由叫. [Me由od] Three commonly used methods for determination of seed vigor ， πC method , the red ink method and the ultraviolet absorp- tion spectrophotometry , were used to deteπnine rye seeds vigor , and distiller water method 国 control. [Result] The results show that there was no si伊ificant difference in determining rye seeds average germination rate between TIC method and the control ηle average germination rate obtaining by the red ink method w田 10.13ì岛 higher than 由at of the control , and there w幽 a significant difference between them. 币le light absorption value of extracts of rye seed after immersed in 8 h under 260 nm had a good correlation 切也 its seed vigor when using ultravio- let absorption spectrophotometry determining its seed vigor , which reflected the rye seedsvigor in a large degree. [Conclusion] The study pro- vides a theoretical basis for production , storage and examination of rye seeds. Key words Rye; seed vigor; Ultraviolet absorption speclI咽photometry; TIC me由od; Red