Spindle dynamics during meiosis in Drosophila oocytes：（主轴动力在果蝇卵母细胞减数分裂期间）.pdfVIP

Spindle Dynamics during Meiosis in Drosophila Oocytes Sharyn A. Endow and Donald J. Komma Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710 Abstract. Mature oocytes of Drosophila are arrested in acute pivoting movement to reorient perpendicular to metaphase of meiosis I. Upon activation by ovulation the oocyte surface. Many oocytes spontaneously com- or fertilization, oocytes undergo a series of rapid plete the meiotic divisions, permitting visualization of changes that have not been directly visualized previ- progression from meiosis I to II. The movements of the ously. We report here the use of the Nonclaret disjunc- spindle after oocyte activation provide new information tional (Ncd) microtubule motor protein fused to the about the dynamic changes in the spindle that occur green fluorescent protein (GFP) to monitor changes in upon re-entry into meiosis and completion of the mei- the meiotic spindle of live oocytes after activation in otic divisions. Spindles in live oocytes mutant for a loss- vitro. Meiotic spindles of metaphase-arrested oocytes of-function ncd allele fused to gfp were also imaged. are relatively stable, however, meiotic spindles of in The genesis of spindle defects in the live mutant oo- vitro–activated oocytes are highly dynamic: the spindles cytes provides new insights into the mechanism of Ncd elongate, rotate around their long axis, and undergo an function in the spindle during the meiotic divisions. eiosis is a specialized cell division that results in localized to multiple foci at the poles of the mouse oocyte M the formation of haploid gametes instead of dip-