电针对去卵巢大鼠下丘脑神经内分泌信号传导的影响论文.docVIP

　　电针对去卵巢大鼠下丘脑神经内分泌信号传导的影响论文 【摘要】 目的：比较正常大鼠性周期不同阶段和电针治疗后去卵巢大鼠下丘脑内侧视前区（medial preoptic area, MPOA）推挽灌流液中的主要神经信息分子的种类和含量，以观察生理和病理状态下，下丘脑神经内分泌信号传导通路的异同，初步探讨电针治疗围绝经期综合征的神经内分泌信号传导机制。方法：用大鼠脑立体定位和核团推挽灌流技术，收集正常雌性大鼠性周期不同阶段的下丘脑MPOA推挽灌流液和去卵巢大鼠电针后的MPOA灌流液［简称针刺灌流液（acupuncture perfusate, AP）］，将不同的灌流液分别微量注射到去卵巢大鼠的MPOA，观察它们各自对去卵巢大鼠阴道脱落细胞和血清雌二醇（estradiol, E2）水平的影响。用放射免疫法和高效液相色谱法测定各组灌流液中促性腺激素释放激素（gonadotropinreleasing hormone, GnRH）、多巴胺（dopamine, DA）、γ氨基丁酸（gammaaminobutyric acid, GABA）、谷氨酸（glutamic acid, Glu）、天冬氨酸（aspartic acid, Asp）和β内啡肽（βendorphin, βEP）的含量。对各组灌流液中的这些物质的种类和含量进行比较分析。结果：正常性周期四个不同阶段的灌流液中的DA、GABA、Glu和βEP等神经活性物质的含量不同；去卵巢大鼠MPOA微量注射正常性周期四个阶段的灌流液后，阴道涂片脱落细胞和血清E2含量分别出现类似正常大鼠性周期四个阶段的特征；在去卵巢大鼠MPOA中微量注射针刺灌流液后.freelain nerval information molecules in perfusate from hypothalamic medial preoptic area (MPOA) of the rats in different sexual cycles and the ovariectomized rats treated by electroacupuncture, so as to observe the similarities and differences of hypothalamic neuroendocrine signal transduction pathechanisms of neuroendocrine signal transduction of electroacupuncture therapeutic effect in perimenopausal syndrome. Methods: The stereo localization technique and pushandpull perfusion of the rat brain nucleus ic MPOA perfusate of the female rats al sexual cycle, and also for collecting the MPOA perfusate of ovariectomized rats after electroacupuncture treatment as acupuncture perfusate (AP). After being respectively microinjected into MPOA of the ovariectomized rats,.freel estradiol (E2) level one (GnRH), dopamine (DA), γaminobutyric acid (GABA), glutamate (Glu), aspartate (Asp) and βendorphin (βEP) in the perfusate of each group munoassay or high performance liquid chromatography, and then the varieties and contents of these substances in the perfusate of each group the rats’ MPOA during different stages of sexual cycle shoe regular changes. After the perfusate icroinjected respectively into the MPOA of the ovariectomized rats, the changes of animal vaginal exf