HPLC-ELSD及紫外分光光度法测定三七中皂苷的 - 天津中草药杂志社.PDFVIP

　 　 2000 31 10 Chinese Traditional and Herbal Drugs ·737· ·· HPLC-ELSD ( 210038)　　江英桥 　王　强　马世平　党学东 　　 Rg 。 CLC-ODS ( 6. 0 1 mm× 150 mm) ,-( 30 ∶70) ,。 ,。 100. 50% ,R SD 1. 82%。 , HPLC,, 101. 50% ,R SD 1. 44%。 。 　　 HPLC-ELSD　 Rg 　　 1 - . Quantitative Determination of Saponins in the Root ofP anax p seudo g inseng var notog inseng by HPLC-ELSD and UV Spectrophotometry 　　China Pharma euti al University ( Nanjing 210038)　 Jiang Yingqiao, W ang Qiang , Ma Shiping and Dang Xuedong 　　 - 1 Abstract A reverse phase HPLC ELSD method for the determination of ginsenoside Rg in the root - . ( ) . of P anax p seud o g inseng var notog inseng Burkill Hoo et Tseng w as reported Chromatographi ondi- : - - ( 6. 0 × 150 ); - ( 30 ∶70) tions Shim pa k CLC ODS olumn mm mm a etonitrile w ater as the mobile phase; Shimadzu LC-6A with SEDEX-55 ELSD dete tor. The method was found to be simple and a urate w ith re overy rate of 100. 50% and R SD= 1. 82% . The established UV spe trophotometri determination . - . of total saponins in P p seud o g inseng var notoginseng was also tried and gave an a urate result oin i- dental w ith that of the HPLC results. The re overy rate w as 101. 50% , and R SD= 1. 44% . It seemed that . - . . both methods an be used reliably for th e quality ontrol of P p seudo ginseng var notog inseng 　　 -