扶正减毒颗粒对放射性肺损伤小鼠肺病理和MMP 2 MMP 9影响的实验研究.pdfVIP

断标准，表明本实验小鼠放射性肺炎造模成功， 2 小鼠照射后的全身表现符合中医“热毒侵肺”致“气阴两虚”“血络淤阻”的证 候传变， 3 扶正减毒颗粒能减轻造模小鼠的放射性肺炎症状，明显改善小鼠的一般情况及 中医证候表现，减轻造模小鼠外周血中红、白细胞的下降。 4 扶正减毒颗粒能减轻造模小鼠活体肺组织肿胀、出血及镜下肺间质充血、出血、 水肿及肺泡结构破坏程度。 5 扶正减毒颗粒可明显抑制小鼠肺组织细胞中 MMP-2、MMP-9 的表达水平，这可 能是中药减轻肺组织结构重塑，从而抑制放射性肺损伤发生发展的机制之一。 关键词 扶正减毒颗粒；放射性肺损伤；MMP-2；MMP-9 Empirical study on changes of lung pathology and the level of MMP-2、MMP-9 of radioactivity injury of mice lungs Abstract Purpose Attenuated by observing the righting of radioactive particles on the lung injury model of mouse lung tissue pathology and MMP-2, MMP-9 and other indicators of the intervention, and to explore the treatment of radioactive particles Fuzheng attenuated lung injury mechanism, clinical use for the drug further theoretical and experimental basis. Methods We chose 60 female Kunming mice were divided into 3 groups: (A group) control group 20: Since the beginning of the experiment day, physiological saline by gastric infusion 0.5ml / (only. D); (B group) simple irradiated group 20: Whole lung irradiation 12Gy + single gastric perfusion by saline 0.5ml / (only. d); (C group) Chinese intervention group 20: whole lung after single exposure to 12Gy + 0.5 particles attenuated gastric perfusion Fuzheng ml / (only. d). Irradiation administered the day to end 42 days after irradiation, 1 day after irradiation, 14 days, 28 days, 42 gifted second broken neck killed at the blank control group, irradiated group and the Chinese intervention in group 5. Each time point to observe the general condition of the body of mice and red blood leukocyte count, said the lung wet weight, gross morphological changes in lung specimens and pathological changes, determined by immunohistochemistry in mice lung cells MMP-2, MMP -9 expression.Experimental data using SPSS18.0 statistical software for statistical analysis. Results 1 the overall observation of animals: the simple irradiation grou