从50mL外周血中高效扩增CIK方法的探讨.pdfVIP

2005 4 12 7 CH IN J CANCER PREV TREAT, April 2005, Vol. 12 No. 7 515 基础临床研究 50 mL CIK 邹征云, 刘宝瑞, 钱晓萍, 杜娟, 王燕 南京大学医学院附属南京鼓楼医院肿瘤科, 江苏 南京210008 New method in vitro for large expansionof CIK cells from 50 mL peripheral blood for clinical therapy ZO U Zheng-y un, L I U Bao-r ui , QIA N X iao-p ing , D U J uan , WA N G Yan Dep artment of On o logy , N anj ing Dr um Tow er H osp ital, Fa ulty of M edi ine , N anj ing University , N anj ing 210008, P. R. China : 寻求有效方法使得一次性采 50 mL 制备 [ ABSTRACT] OBJECTIVE : To est a lish a new 的细胞因子诱导的杀伤细胞( cytokine- induced killer, CIK) method for large expansion of cytokine-induced 足以满足临床过继性细胞免疫治疗需要: 从 50 mL killer ( CIK ) cells from only 50 mL peripheral 外周 中分离外周 单 个核细胞(peripheral lood mononu- lood ( PB) in v itro for clinical immunotherapy. clear cell, PBMC) 后, 分别应用AB 方法进行培养A 培养 METHODS: H uman peripheral lood monocyte 法应用 干扰素( interferon-, IFN-) CD 单克隆抗体 ( PBMC) w ere separated from 50 mL PB of donors 3 ( CD-monoclonal ant i ody, CD mA ) 白细胞介素-2( inter- and cultured in vitro for expansion y tw o differ- 3 3 leukin-2, IL-2) 和植物 球凝集素( phytohemagglutinin, ent methods respectively. T he expansion, killing PH A) 行常规培养; B 培养法在 A 培养法的基础上于培养的 activit y, phenotype changes and cell cycle of CIK 第2 天, 另外加入终浓度为10 ng/ mL 的IL-4, 1 000 U/ mL w ere analyzed to evaluate the new method. RE- 7 8 的粒细胞巨噬细胞集落刺激因子( granulocyte macrophage SU LTS: 5 10 - 1 10 P