免疫学-NKT-2016课件.pptxVIP

;淋巴细胞;CLP;自然杀伤细胞（Natural Killer Cells）;Lymph node;NK细胞杀伤靶细胞的作用机制;NK 对靶细胞的识别;NK细胞活化模型;KIR与KAR的信号传导;KIR对KAR信号的抑制;NK受体的结构;Ly49;KIR;NKG;NCR(Natural Cytotoxicity Receptor);;Microbes and Infection 4 (2002) 1545–15;TRENDS in Immunology Vol.28 No.6;THE LANCET ? Vol 356 ? November 25, 2000;NK 细胞的功能—免疫调节;NK 细胞肿瘤免疫治疗;MHC I Mismatch;CAR-NT;;;;A unique subpopulation of T lymphocytes (5%) that coexpressed TCR and some markers traditionally associated with NK cells Express multiple NK markers such as NK1.1 antigen (NRK-P1/CD161 in humans) Express αβ TCR. Like conventional T cells, NKT cells express T-cell receptors that are generated via somatic DNA rearrangement. ;Mature NKT cells are distributed widely, including in the bone marrow, gastrointestinal tract and skin, etc In humans, only 0.2% of peripheral blood T cells are NKT cells. they are enriched in the omentum (网膜), where they represent approximately 10% of T cells In mouse, they represent approximately 1–2% and 20–30% of lymphocytes in the spleen and liver ;T CELL RECEPTOR EXPRESSION;ANTIGENS RECOGNITION: CD1d;NKT: Development;CD1d ligand;;Activation of NKT cells;NKT cell subsets;;;;组成性表达TCR γδ-CD3 复合物和CD2 分子 位于黏膜上皮的γδ-T 细胞占局部T 细胞总数的50%，表型多为CD8 单阳性； 外周血中γδ-T 细胞仅占成熟T 细胞的2%～7%，表型为CD4 和CD8 双阴性。 在脾脏和其它各器官均有分布 ;γδTCR的基因结构;γδT细胞的发育;T-cell receptors (TCR);γδTCR具有有限的多样性;;分布于黏膜上皮内的亚群，其TCR 多样性非常有限（Vd1）。 分布于外周血的亚群，其TCR 90%以上为Vγ9Vδ2 。 分布于淋巴组织的亚群，其TCR 具有多态性 ;γδ T细胞识别的抗原;蛋白抗原;蛋白抗原;磷酸化抗原;磷酸化抗原;gdT细胞的活化;gdT细胞的生物活性;;gdT细胞的生物活性;gdT细胞的生物学作用-抗感染;gdT细胞的生物学作用-皮肤;gdT细胞的生物学作用-抗肿瘤;gdT cell based cancer immunotherapy;gd T细胞summary