高产抗真菌抗生素Ustilagoesculenta菌株的选育.pdf

贵州大学 2009 届微生物学硕士论文： 高产抗真菌抗生素 Ustilagoesculenta菌株的选育 5.2 展望······························································································· - 42 - 主要参考文献······························································································ - 44 - 致 谢······································································································· - 47 - 附 录 Ⅰ···································································································- 48 - 附 录 Ⅱ···································································································- 49 - b 贵州大学 2009 届微生物学硕士论文： 高产抗真菌抗生素 Ustilagoesculenta菌株的选育 摘 要 传统食用担子菌 Ustilagoeseulenta菌株可代谢产生一种对部分人体病原真 菌、部分植物病原真菌等具有较强拮抗作用的抗真菌抗生素。该抗生素具有安全、 稳定、抗菌谱广等特点，为开发和生产新型农用抗真菌抗生素提供了可能。 自茭白分离 38 株菌株。综合形态学和分子生物学方法，共鉴定了 33 株菌株 为 U.esculenta菌株。以 Aspergillesnigersp.为拮抗菌，琼脂块法测定分离菌株的 抑菌活性，筛选到抑菌活性较强的分离菌株 UEZC。 为筛选高产抗真菌抗生素菌株，以 UEZC 作为出发菌株，经 60Co-γ射线辐 射诱变，以拮抗菌对峙法初筛，琼脂块法、管碟法复筛，筛选到较高产抗真菌抗 生素突变株 MUE-4，其抑菌活性较 UEZC 菌株提高了近 1 倍，遗传稳定性良好。 经 ITS1-5.8S rDNA-ITS2 基因序列分析，高产突变株 MUE-4 较其出发菌株 UEZC 在 ITS1-5.8S rDNA-ITS2 区段发生了较为明显的变异。 采用响应面（RSA）法对诱变选育得到的高产菌株 MUE-4 进行发酵工艺的 初步研究，在所获得的最优发酵条件下，抑菌活性较优化前提高了 46%，较诱变 出发菌株 UEZC 提高了 194%。 关键词：Ustilagoesculenta；抗真菌抗生素；鉴定；ITS1-5.8S rDNA-ITS2； 60Co-γ射线；诱变育种 I 贵州大学 2009 届微生物学硕士论文： 高产抗真菌抗生素 Ustilagoesculenta菌株的选育 Abstract Abstract AAbbssttrraacctt The traditional edible basidiomycetes Ustilagoeseulentastrain was an antifungal antibiotics producing strain. The antifungal antibiotics that UEZC produced had strong anta