精子DNA完整性与宫腔内人工授精妊娠率关系的相关研究.pdfVIP

The Study of the Relationship Between Sperm DNA Fragmentation Index and Intrauterine Insemination Pregnancy Rate Abstract:Objective: Sperm DNA is an important parameter of sperm quality, foreign studies have shown that sperm DNA have obvious lili relation with sperm morphology and function.Sperm DNA is an important facnotor that could cause infertility and miscarriage.This experimentdafang yidian has an exploration in the relationship between sperm DNAdafangni d xing damage and the intrauterine insemination pregnancy rateyida by using acrimeiou wen wo dine orange test(AOT) to refer different degree of sperm DNA damagbuyao guane to provide theoretical support for different infertile patientcanjia shenm s to choose different assisted reproductive technology.PureSperm densityzhongji gedou -gradient centrifugatiomeiyoukissing n is one of the most popular semen optimiyoudian xiang zation processing methods.Thiget outs study is to analyze the influence PureSpshuaijiao erm density-gradient centrifugation adating ct on sperm DNA and other sperm pyemanarameters by testing sperm DNA and otherboth parameters before and after sisa the optimization processing method. Methods: 46you semen samples were obtmeiyou yuansained from 36 male attending medi dong ical reproduction center of affiliatekeyi chule cuoyanj d hospital of Luzhouno way Medical College forthieng intrauterine insemination froyugoushou m Octburan ober 2011 to March 2012. After rmenaoutine semen analysis, Diff-xieixe quick yazi xiwngz staining and AOT, each sample processeso youed Pure Sperm density-shenm weidao gradient centrireallyfugation, then do routine semen analysis, Ddazhanshenshou iff- quick staining and AOT againtogether. Tundre he changes of sperm DNA 、 nxiwang ni ormal sperm morphology rate and sperm motility rate before and after the tremens yeshi atment and the relationgkeneng ship between sperm