086班18号陈静决明子总黄酮提取工艺的响应面设计优化精要.doc

决明子总黄酮提取工艺的响应面设计优化 摘要 本文以为材料Y与芦丁质量浓度X的关系为：Y=0.0951X+0.0012，相关系数r=0.9997，表明黄酮含量在20~70μg/mL之间浓度与吸光度呈良好的线性关系。 响应面实验设计方法（RSD）最佳工艺参数为结果表明 CASSIA SEED FLAVONOIDS EXTRACTION PROCESS RESPONSE SURFASE DESIGN OPTIMIZATION ABSTRACT In this paper it for the materials, in the single factor analysis, on the basis of flavonoids in rate for response value, the study by the response surface experimental design method (RSD) extraction definitely the flavonoids process conditions. Establish the concentration of rutin-spectrophotometry the regression equation of the relationship to absorbency Y and the relationship between the mass concentration rutin X for: Y = 0.0951 X + 0.0012, correlation coefficient r = 0.9997, the results show that the content of flavonoids in 20 to 70 μ g/mL concentration and absorbency between a good linear relationship between. The response surface experimental design method (RSD) best process parameters for ethanol concentration 56.1%, extraction temperature of 80 ℃, reaction time is 3.6 h, the liquid-solid ratio 20.6, extraction 1, the process conditions can make the total yield of 3.56%. The results show that the process conditions can provide reference for industrial production data. The response surface method in experimental design can be continuous range for analysis, better than now widely used to only analysis of discrete conditions of orthogonal experiment design. Key words: The response surface; Cassia seed; flavonoids  目 录 1前言--------------------------------------------------------------------------------------1 1.1决明子-----------------------------------------------------------------------1 1.1.1 决明子的植物学特征-----------------------------------------------------------------1 1.1.2 决明子的药用价值--------------------------------------------------------------------1 1.2 黄酮类化合物的简介----------------------------------------------------------------------2 1.2.1 黄酮类化合物-------------------------------------------------------------2