精索静脉曲张和精液质量的关系.pdfVIP

international journal of andrology ISSN 0105-6263 ORIGINAL ARTICLE Effect of microsurgical varicocelectomy on human sperm chromatin and DNA integrity: a prospective trial A. Zini, R. Azhar, A. Baazeem and M. S. Gabriel Division of Urology, Department of Surgery, Royal Victoria Hospital, McGill University, Montreal, QC, Canada Summary Keywords: There is evidence from retrospective studies that varicocelectomy can improve IVF, male infertility, nuclear compaction, sperm DNA damage in infertile men with a clinical varicocele. The objective sperm DNA fragmentation, sperm motility, of this prospective study was to examine further the effect of varicocelectomy varicocele on sperm chromatin and DNA integrity. We evaluated a consecutive series of infertile men (n = 25) who underwent microsurgical varicocelectomy for Correspondence: Armand Zini, St Mary’s Hospital, 3830 treatment of clinical varicocele. We examined conventional sperm parameters Lacombe Ave., Montreal, QC, Canada H3T and sperm chromatin structure assay parameters (percentage DFI – DNA 1M5. E-mail: ziniarmand@ fragmentation index and percentage HDS – high DNA stainability, an index of chromatin compaction) before and 4 and 6 months after microsurgical Received 21 September 2009; revised 27 varicocelectomy. Sperm DNA integrity improved significantly after surgery November 2009; accepted 15 December (percentage DFI decreased from 18 ± 11% before surgery to 10 ± 5%, and 2009 7 ± 3%, at 4