烟草染色体倍性快速鉴定方法.pdfVIP

业生物技术学报 Journal of Agricultural Biotechnology 2006,14 (2): 255~258 ·研究论文· 烟草染色体倍性快速鉴定方法* 1，2 1 1 惠琴 , 张宪银 , 薛庆中 ** (1. 浙江大学农业与生物技术学院，杭州 310029; 2. 青海大学农牧学院，西宁810003) 摘要: 对两个杂交组合(G28 NC2326 和K326 Coker 176) F1 烟草( ) 花药培养诱导出763 个不同倍性 植株。基 植株的花和种子结实率常规鉴定倍性水平比较，用气孔保卫细胞叶绿体数目预测植株倍性准确率达93.52% 。表明 采用气孔保卫细胞叶绿体计数法可以在苗期快速、准确地确定植株染色体倍性。同时, 在移栽前间接地鉴定、筛选出叶绿体数 14 的单倍体苗，作进一步秋水仙碱加倍处理，以减少对单倍体材料的浪费，加快DH 群体构建速度。 倍性； ； 关键词: 烟草； 叶绿体 DH 群体 中图分类号: S184 文献标识码: A 文章编号: 1006-1304(2006)02-0255-04 Rapid Determination of Ploidy Level of Chromosome in Tobacco( ) ZHU Hui-qin1，2 1 1 , ZHANG Xian-yin , XUE Qing-zhong ** ( ) The ploidy level of 763 anthers deirived from both crosses (G28 NC2326 and K326 Coker 176) of tobbaco ( ) were determined. The result of identification by counting the number of stoma chloroplast in guard cell was consistent with those observations of flower and seed fertillity of the plants, with the average accurate rate of 93.52%, showing that measurement of the number of stoma chloroplast in guard cell could be considered as a fast and accurate method to determine haploid, diploid or poly- ploid of tobacco in the seedling stage. In order to reduce the waste of haploid plants and speed up establishment DH pop