KLF3基因3′-UTR区双荧光素酶报告载体及其突变载体的构建与活性鉴定.pdfVIP

2017 44 3 644650 China A nim al H usbandry Vete r inary M e d ic ine doi cnki j K L F3 3 U TR KL FU T R K L F miRNA s PCR K L F U T R X ho N ot T argetscan K L F U T R miRNA miRNA s mimics K L F U T R T K L F U T R miR miR mimics K L F U T R P K L F U T R miR K L F K L F miR Q A Construction and Activity Identification of Dual Luciferase Reporter Vector and Mutation Vector of KLF3 Gene 3 UTR Z HAI Bo LI Xu WANG Chunxin Z HAO Yunhui SUN Ming Z HANG Lichun Z HANG Mingxin Sheep Breeding L aboratory B ranch of A nimal H usbandry Jilin A cademy of A g ricultural Sciences Gongz huling China Tonghua Teachers College Branch Tonghua China Abstract T his study w as aimed to construct the dual lucif erase reporter vector w hich contained K L F ene U T R and it s mutation vector and analyze the possible miRNA s w hich could regu g late K L F ene ex pression In this study T he K L F ene U T R w as amplified by poly merase g g chain reaction PCR and inserted in the dual lucif erase reporter vector w hich w as digested by X ho and N ot With T arget scan to predict the miRNA s w hich might interact w ith K L F gene U T R T cells w as treated w ith recombinant vector or it s mutation vector or empty vector and miRN A mimics or control by Lipof ectamine T he dual lucif erase reporter assay sy stem w as used to compare