张新祥《生化分析》酶的电化学 张淑冬 刘王莲.pptxVIP

酶固定化技术及 酶的生物传感器;;Fig. 1 Scheme of a biosensor.;固定化酶的优点：;酶固定化的载体：;Fig. 2 Schematic representation of the main different methods of enzyme immobilization. E: enzyme, P: inert protein.;包埋（entrapment）：; 聚糖：藻朊酸盐、琼脂 ;吸附（adsorption）：;分子间交联（cross-linking）：;Fig.4 Enzyme immobilization on carboxylated surface by carbodiimide coupling (A) without or (B) with NHS.;Fig. 5 Enzyme immobilization on an aminated surface by (A) carbodiimide or (B) glutaraldehyde coupling.;Fig. 6 Schematic representation of the reversible immobilization of histidine-tagged biomolecules to an electrogenerated poly(pyrrole)-NTA ?lm.;酶的固定化技术;酶的固定化技术;Fig. 9 Immobilization of non-pure enzymes on nonporous supports: the case of a rapid immobilization of the contaminant enzymes.;Fig. 10 Immobilization of non-pure enzymes in porous supports: the case of large contaminant proteins.;Fig. 11 Use of hererofunctional supports for the one step immobilization and purification of enzymes.;Fig. 12 Magnetic nanoparticles for one step immobilization-purification of poly-His tagged proteins.;酶固定化的应用：;应用;葡萄糖酶电极;葡萄糖酶电极;葡萄糖酶电极;第一代葡萄糖酶电极;第二代葡萄糖酶电极;第二代葡萄糖酶电极;第三代葡萄糖酶电极;第三代葡萄糖酶电极;第三代葡萄糖酶电极;第三代葡萄糖酶电极;HIV抗体电化学酶联免疫吸附传感器;HIV抗体电化学酶联免疫吸附传感器;总结;35; ‘X0\P-