论文——阿霉素磁性介孔氧化硅纳米颗粒靶向治疗口腔鳞状细胞癌细胞的体外实验研究.doc

阿霉素磁性介孔氧化硅纳米颗粒靶向治疗口腔鳞状细胞癌的体外实验研究 esoporous Magnetic Silia Nanoparticles,DOX@M-MSNs）对口腔鳞状细胞癌细胞的体外靶向杀伤作用。本研究使用甲基噻唑基四唑（MTT）法，该方法的基本原理便是利用流式细胞仪等设备仪器来对DOX@M-MSNs对于CAL-27的体外靶向抗肿瘤效果进行科学合理的检测。最终结果表明，单纯磁性介孔氧化硅纳米颗粒的生物相容性是非常良好的，尽管浓度值为100ug/ml，但是细胞仍然具有非常高的存活率。当作用时间有所增长或者浓度值有所提升的时候，相较于单纯阿霉素原料组，DOX@M-MSNs对于肿瘤细胞的增殖抑制作用要明显强得多，差异有统计学意义（p0.05）。并且，测试结果最终还同时表明DOX@M-MSNs能够在很大的程度上促使肿瘤细胞的凋亡，与单纯阿霉素相比较，其凋亡率往往更高，同时当药物的作用时间延长的时候，其对肿瘤细胞的抑制效果也在很大的程度上有所提升，其凋亡率也大大的提升。激光共聚焦显微镜检测结果显示通过外加磁场，磁性区域的DOX@M-MSNs于肿瘤细胞的聚集优于非磁性区域。最终的研究结果表明，DOX@M-MSNs能够选择性地进到肿瘤细胞里面，从而使得阿霉素对肿瘤细胞的毒性有所增强，进而使得其杀伤肿瘤细胞的效果显著提高，与此同时，外加的磁场可以显著地提升输送DOX@M-MSNs进到肿瘤细胞里面的效率，进而使得肿瘤抑制效果大大提升。 【关键词】阿霉素；介孔氧化硅纳米颗粒；外部磁场；靶向作用;肿瘤 Application of targeted therapy of oral squamous cell carcinoma using Doxorubicin-loading Mesoporous Magnetic Silia Nanoparticles in vitro 【Abstract】Objective To investigate the targeting effect of Doxorubicin-loading Mesoporous Magnetic Silia Nanoparticles on oral squamous carcinomas cells in vitro.Method Methyl thiazolyl tetrazolium (MTT)，flow cytometry and laser scanning confocal microscope assaies used to detect the targeted anti-tumor activity of DOX@M-MSNs against CAL-27 .Results The blank Mesoporous Magnetic Silia Nanoparticles showed excellent biocompatibility with cell viability 80% at the maximum tested concentration. With increase of concentration and the going of time,The DOX@M-MSNs on tumor cell proliferation inhibition was superior to that of free DOX?and showed significant?difference (p0.01). Flow cytometry showed that DOX@M-MSNs induce heiger necrotic rates than free DOX ,and with the going of time, the necrosis rate increased. Laser scanning confocal microscope showed that the magnetic area of DOX @ M-MSNs in gathered is better than that of non-magnetic regions of the tumor cells through external magnetic field, with increase of time, compared with non magnetic area, the magnetic area showed a higher cell death .Conclusion DOX@M-MSNs can selectivity recognize oral squamous carcinomas cells , ?enhanc