跨物种扩增筛选获得蓝马鸡(Crossoptilon auritum)微卫星多态分子标记(英文).pdfVIP

Chinese Birds 2012, 3(2):103–107 SHORT COMMUNICATION DOI 10.5122/cbirds.2012.0010 A panel of polymorphic microsatellites in the Blue Eared P heasant (Crossoptilon auritum) developed by cross-species amplification Langyu GU 1,Yang LIU 2,*, N?ng?ANG 1,Zhengwang ZHANG 1,? 1 MOE Key Laboratory for Biodiversity Sciences and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing 100875, China 2 Computational and Molecular Population Genetics, Institute of Ecology and Evolution, University of Bern, Baltzerstrasse 6, 3012, Bern, Switzerland Abstr act Polymorphic microsatellites are among the versatile genetic markers in molecular ecology studies. In contrast to de novo isolation of microsatellites from target species, cross-species ampli?ca- tion is a cost-effective approach for a fast development of microsatellite markers from closely related taxa. In our study, we cross-ampli?ed a panel of polymorphic microsatellite markers for the Blue Eared Pheasant (Crossoptilon auritum), a species endemic to China of considerable conservation con- cern. We obtained 11 polymorphic microsatellite markers selected from 112 candidate loci, originally isolated from other Galliforme species. This panel of makers has shown moderate to high levels of polymorphism and include a Z-chromosomal linkage locus. We carried out preliminary analyses of parentage among captive individuals with a known pedigree using this new panel of microsatellites. Our results suggest that the high utility of these markers may be powerful tools for studies in conser- vation genetics of eared-pheasants and other endangered Galliforme species. Keywords Crossoptilon auritum, microsa