基于线粒体D-loop序列的白头鹤越冬种群遗传结构研究(英文).pdfVIP

Chinese Birds 2012, 3(2):71–81 ORIGINAL ARTICLE DOI 10.5122/cbirds.2012.0012 Genetic structure of wintering Hooded Cranes (Grus monacha) based on mitochondrial DNA D-loop sequences Lili ZHANG, Lizhi ZHOU ? , Yanli DAI School of Resources and Environmental Engineering, Institute of Biodiversity and Wetland Ecology of Anhui University, Hefei 230601, China Abstr act The Hooded Crane (Grus monacha) is a waterbird wintering in the wetlands of the middle and lower reaches of the Yangtze River, China. The gradual habitat loss resulting from wetland deg- radation may have posed negative effects on the structure of our wintering populations. For its effec- tive protection, it is important to conduct an intensive study on the genetic structure of this popula- tion. A total of 221 faecal samples, nine feather samples and four muscle samples of Hooded Cranes from four wintering populations, i.e., from Caizi Lake and Shengjin Lake in Anhui, Poyang Lake in Jiangxi and Chongming Dongtan in Shanghai, were collected for this study. Full-length 1103–1104 bp mtDNA D-loop sequences from 72 samples were ampli?ed using PCR. Based on our ampli?ed D-loop sequences and the sequences of two individual birds obtained from GenBank (AB017625 and AB023813), we analyzed the genetic structure of these four wintering Hooded Crane populations. Twenty six variable sites were found among 72 target sequences in the four wintering populations and 23 haplotypes were de?ned. Genetic diversity analyses showed that the haplotype diversity of Hooded Cranes was 0.823 0.042 with a nucleotide diversity of 0.00157 0.00021. The F values of ± ± ST