杀虫剂压致死剂量对褐飞虱雄虫的生殖效应.docVIP

杀虫剂亚致死剂量对褐飞虱雄虫的生殖效应 摘要：褐飞虱是由农药引起再猖獗的典型害虫。在中国以及其他亚洲国家，褐飞虱的大发生主要与滥用杀虫剂以及对吡虫啉产生抗性有关。菊酯类、有机磷类（三唑磷）农药的使用可能会诱导褐飞虱种群的再猖獗。然而，对农药诱导的害虫再猖獗机制的研究主要集中在刺激雌虫生殖方面，完全忽视了雄虫在生殖中的作用。本实验研究了三唑磷和溴氰菊酯对褐飞虱雄性附腺蛋白含量的影响。结果表明,当褐飞虱3龄时用杀虫剂处理，交配状况、杀虫剂种类、浓度都显著影响褐飞虱雄性附腺中蛋白质含量。用三唑磷处理后褐飞虱交配前后的蛋白质含量都高于对照，用溴氰菊酯处理后，交配前的蛋白质含量高于对照，而交配后的含量低于对照。两种杀虫剂高浓度处理后雄性附腺蛋白含量都高于低浓度处理的。交配状况以及杀虫剂种类同样会显著影响着5龄处理的褐飞虱雄性附腺中蛋白质的含量。杀虫剂处理后，交配前的雄性附腺蛋白含量显著高于对照。相反，交配后的蛋白含量显著低于对照，而且不同的交配组合也影响雄性附腺中的蛋白质含量。本实验研究结果为褐飞虱再猖獗机制提供了有力论据。 关键词：褐飞虱，杀虫剂，雄性附腺，蛋白质 Abstract: The brown planthopper, Nilaparvata lugens (St?l) (Homoptera: Delphacidae), is a classical resurgent pest induced by insecticides. Resent outbreak of N. lugens that occurred in China and other Asian countries has been mainly associated with pesticide overuse and resistance to imidacloprid. Use of synthetic pyrethroids and organophosphates (triazophos) is likely to induce the population resurgence. However, investigation on resurgent mechanisms mainly focuses on stimulation of reproduction of adult female induced by insecticides, effect of insecticide-induced male reproduction on resurgent mechanisms of N. lugens has not been understood so far. The present investigation examined changes of protein leve the male accessory gland (MAG) induced by two insecticides triazophos and deltamethrin. The results showed that male mating, insecticide types and their concentrations significantly affected protein content in MAG of N. lugens adult male developed from third instar nymphs feeding on treated rice plants. Protein contents in MAG both before and after mating for triazophos treatment, and before mating for deltamethrin one were higher than those for controls, however, those after mating for deltamethrin one were lower than those for control. Protein content in MAG under high concentrations of two insecticides was significantly higher than that under low ones. Male mating and insecticide types also significantly affected protein content in MAG of N. lugens adult male developed from fifth