人参皂苷Rg3对小鼠B16黑素瘤_省略_侵袭_转移及MMP_9表达的影响.pdfVIP

肿瘤2011年2月第31卷第2期 Tumor Vol. 31, February, 2011 117 DOI:10.3781/j.issn.1000-7431.2011.02.005 Basic Research·基础研究 人参皂苷Rg3对小鼠B16黑素瘤细胞侵袭、转移及MMP-9表达的影响 姜 新1，辛 颖2，许天敏3，曲雅勤1 1. 吉林大学第一医院肿瘤中心放疗科，长春 130021；2. 吉林大学病理生物学教育部重点实验室，长春 130021； 3. 吉林大学第二医院妇产科，长春 130021 [摘要] 目的：观察人参皂苷Rg3（简称Rg3）对小鼠黑素瘤细胞B16的肺转移、侵袭能力及基质金属蛋白酶-9 （matrix metalloproteinase-9，MMP-9）表达的影响，探讨其抗肿瘤转移的作用机制。方法：体内建立小鼠自发肺 转移和实体瘤模型，观察腹腔注射不同剂量的Rg3（对照组给予0.9％氯化钠溶液）后，肺部肿瘤转移灶的数目， 并检测实体瘤中MMP-9蛋白的表达情况。采用Boyden小室侵袭实验及免疫细胞化学染色法检测Rg3对肿瘤细胞体 处侵袭能力及MMP-9表达的影响。结果：采用不同剂量的Rg3（0.3、1.0和3.0 mg/kg）治疗后，小鼠肺部转移灶 的数目均较少，肿瘤组织中MMP-9的表达水平降低，与对照组比较差异有统计学意义（P ＜0.05）。在体外，2.5 和5.0 μg/mL Rg3治疗组中侵袭穿过人工基膜的B16细胞数目明显少于对照组（P＜0.01），且5.0 μg/mL Rg3可抑 制肿瘤细胞中MMP-9的表达。 结论：Rg3能够抑制小鼠黑素瘤细胞的肺转移，其抗肿瘤转移作用可能是通过降低 肿瘤细胞中MMP-9的表达水平及细胞侵袭能力来实现的。 [关键词] 黑素瘤，实验性；人参皂苷类；肿瘤转移；肿瘤浸润；基质金属蛋白酶 [中图分类号] R739.5 [文献标志码] A [文章编号] 1000-7431（2011）02-0117-05 Effects of ginsenoside Rg3 on the invasion and metastasis of mouse melanoma cell line B16 as well as the expression of MMP-9 JIANG Xin1, XIN Ying2, XU Tian-min3, QU Ya-qin1 1. Department of Radiotherapy, Cancer Center, First Hospital; 2. Ministry of Education Key Laboratory of Pathobiology; 3. Department of Obstetrics and Gynecology, Second Hospital, Jilin University, Changchun 130021, China [ABSTRACT] Objective: To observe the effects of ginsenoside Rg3 on the invasive and metastatic potentials of mouse melanomas B16 cells in vitro and in vivo, as well as the expression of matrix metalloproteinase-9 (MMP-9), and to investigate the underlying mechanisms. Methods: The grafted melanoma and its spontaneous lung metastasis model in mice was established by transplanting with B16 cells. The number of metastatic lesions in the lung was calculated, and the expression of MMP-9 in melanoma tissues was detected b