3种不同提取方法对蜘蛛香挥发油化_省略_成分的气相色谱_飞行时间质谱分析_张敏_.pdf

· 3312 · 当代医药论丛杂志 ·研 究 报 告· 3种不同提取方法对蜘蛛香挥发油化学成分的 气相色谱-飞行时间质谱分析 张敏，赵梅，印酬，龙厚宁，孟小夏，王鹏娇，高秀丽 （贵州医科大学药学院，贵阳 550025） 摘要：目的：比较不同提取方法所得蜘蛛香挥发油化学成分的差异。方法：采用水蒸气蒸馏、超临界CO2萃 取及索氏提取蜘蛛香挥发油，并用气相色谱-飞行时间质谱联用技术分析其化学成分，以峰面积归一化法计算各 成分的相对含量。结果：3种提取方法所得蜘蛛香挥发油的化学成分存在一定差异，从水蒸气蒸馏提??蜘蛛香挥 发油中鉴定出96个组分，超临界CO2萃取中鉴定出67个组分，索氏提取鉴定出66个组分。结论：3种提取方法所得 蜘蛛香挥发油中异戊酸均为含量最高的成分，水蒸气蒸馏提取大多为萜类化合物，超临界CO2萃取和索氏提取主 要为酸类和缬草素类。 关键词：蜘蛛香；水蒸气蒸馏；超临界CO2萃取；索氏提取；气相色谱-飞行时间质谱；挥发油；相对含量 基金资助：贵阳市科技计划项目（No.筑科合同[2012204]号） GC-TOFMS analysis of volatile oil extraction form Valeriana jatamansi Jones. by three different methods ZHANG Min, ZHAO Mei, YIN Chou, LONG Hou-ning, MENG Xiao-xia, WANG Peng-jiao, GAO Xiu-li ( School of Pharmacy, Guizhou Medcial University, Guiyang 550025, China ) Abstract: Objective: To compare the differences in chemical composition of volatile oil extraction form Valeriana jatamansi Jones by three methods. Methods: The volatile oil was extracted from Valeriana jatamansi Jones. using 3 different methods including steam distillation extraction, supercritical CO2 extraction and Soxhlet extraction, and then the chemical compositions in volatile oil extraction were analyzed by GC-TOFMS. Finally, the peak area normalization method was used to calculate relative content of each component. Results: There were some differences in the chemical composition of three kinds of extraction methods of the volatile oil from Valeriana jatamansi Jones.. The results of GC-T