牛磺酸联合脐带间充质干细胞移植修复酒精性肝损伤.docVIP

牛磺酸联合脐带间充质干细胞移植修复酒精性肝损伤 李海涛1，陈 超2(河北省遵化市人民医院，1外一科，2病理科，河北省遵化市 064200) 引用本文：李海涛，陈超. 牛磺酸联合脐带间充质干细胞移植修复酒精性肝损伤[J].中国组织工程研究，2017，21():87-91. DOI: 10.3969/j.issn.2095-4344.2017.01.016 ORCID: 0000-0002-9599-1323(李海涛) 文章快速阅读： 文题释义： 牛磺酸：又称β牛黄中分离出来，纯品为无色或白色斜状晶体，无臭，牛磺酸化学性质稳定，不溶于乙醚等有机溶剂，是一种含硫的非蛋白氨基酸，在体内以游离状态存在，不参与体内蛋白的生物合成。牛磺酸虽然不参与蛋白质合成，但它却与胱氨酸、半胱氨酸的代谢密切相关。人体合成牛磺酸的半胱氨酸亚硫酸羧酶活性较低，主要依靠摄取食物中的牛磺酸来满足机体需要。 细胞色素氧化酶P450(cytochrome P450, CYP450)超家族：是重要的生物转化酶系，根据1993年Nelson等科学家制定的，根据P450分子的氨基酸序列的统一命名法，CYP450可以被进一步分为几个族。涉及大多数药物代谢的CYP450酶系主要有CYP1，CYP2，CYP3 3个族，CYP1A2酶亚家族主要在肝脏表达，占体内CYP450酶总量的13%，仅次于CYP3A酶和CYP2C酶，居第3位。代谢临床5%-10%的常规应用药物，故CYP1A2酶的活性与许多药物的疗效或毒性相关，其还参与多种与癌症发生密切相关的化学性毒物(如亚硝胺)和内源性激素的代谢，具有重要的药理学和毒理学意义。CYP1A2酶基因多态性和表型差异的研究，可用于评价临床药物治疗效果。 Taurine combined with umbilical cord blood mesenchymal stem cell transplantation for liver injury repair Li Hai-tao1, Chen Chao2 (1Department of Surgery, 2Department of Pathology, Zunhua People’s Hospital, Zunhua 064200, Hebei Province, China) Abstract BACKGROUND: Studies have shown that umbilical cord mesenchymal stem cells (UC-MSCs) can be induced to differentiate into liver cells, playing a role in liver repair. OBJECTIVE: To explore the effect of taurine combined with UC-MSCs transplantation on liver injury repair. METHODS: Forty healthy Sprague-Dawley rats were used to make chronic alcoholic liver injury models through intragastric administration of 56 degrees liquor, and then randomized into model group, UC-MSCs group and combined treatment group (taurine combined with UC-MSCs transplantation). Model rats were given portal vein injection of 1 mL normal saline in the model group, 1 mL UC-MSCs suspension in the UC-MSCs group, and 0.28 g/L taurine plus 1 mL UC-MSCs in the combined treatment group. Two weeks after transplantation, levels of serum superoxide dismutase, glutathione peroxidase, catalase, alanine aminotransferase, malondialdehyde, endotoxin and interleukin-4 were detected. RT-PCR was used to detect CYP