COMPARISON OF THE GROWTH OF LACTOBACILLUS.pdfVIP

SC IE N TI AR UM POLONO R U MACTA Acta Sci. Pol., Technol. Aliment. 7(2) 2008, 5-20 Corresponding author – Adres do korespondencji: Dr in?. Kamila Goderska, Institute of Food Technology of Plant Origin of Life Sciences in Poznań, Wojska Polskiego 31, 60-624 Poznań, Poland, e-mail: kamilag@up.poznan.pl COMPARISON OF THE GROWTH OF LACTOBACILLUS ACIDOPHILUS AND BIFIDOBACTERIUM BIFIDUM SPECIES IN MEDIA SUPPLEMENTED WITH SELECTED SACCHARIDES INCLUDING PREBIOTICS Kamila Goderska, Jacek Nowak, Zbigniew Czarnecki University of Life Sciences in Poznań Abstract. The performed investigations evaluated the growth dynamics of Lactobacillus acidophilus DSM 20079, Lactobacillus acidophilus DSM 20242, Bifidobacterium bifidum DSM 20082, Bifidobacterium bifidum DSM 20215, Bifidobacterium bifidum DSM 20239, Bifidobacterium bifidum DSM 20456 in media supplemented with various saccharides, including prebiotic preparations. The addition of saccharides to the medium exerted a sig- nificant influence not only on the number of bacterial cells but also on their acid-creating capability. Glucose, lactose and saccharose turned out to be the easiest available saccha- rides for all the bacterial strains tested. In the media supplemented with these sugars the highest numbers of bacterial cells were determined. At the shortage of mono- and disac- charides, all strains of the bacteria tested were capable of utilising the prebiotic prepara- tions as sources of carbon and energy in the media. The amount and isomeric forms of lactic acid produced by Lactobacillus acidophilus DSM 20079 and Bifidobacterium bifi- dum DSM 20239 were determined. Both strains meet the requirements adopted by the WHO and produce more than 70% lactic acid L(+) in the media with the addition of vari- ous saccharides. Lactobacillus acidophilus DSM 20079 was found to produce signifi- cantly higher amount of lactic acid in different media. Key words: probiotics, prebiotic, Lactobacillus acidophilus, Bifid