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http://www.TurkJB ISSN 1303–829X (electronic) 0250–4685 (printed) 344 Comparison of Three Methods for Measurement of HbA1c [HbA1c ?l?ümünde Farkl? ü? Y?ntemin Kar??la?t?r?lmas?] Technical Report [Y?ntem Bildirisi] Türk Biyokimya Dergisi [Turkish Journal of Biochemistry–Turk J Biochem] 2010; 35 (4) ; 344–349. Yay?n tarihi 15 Aral?k, 2010 ? TurkJB [Published online 15 December, 2010] Fatih ?z?elik1, ?mer Yi?iner2, Muhittin A. Serdar3, ?smail Kurt3, Muzaffer ?ztosun1, Erol Arslan4, Alev Orhun5 1 Gümu?suyu Military Hospital, Laboratory of Clinical Biochemistry, Istanbul - Turkey 2 GATA Haydarpa?a Training Hospital, Department of Cardiology, Istanbul - Turkey 3 Gülhane School of Medicine, Department of Clinical Biochemistry, Ankara - Turkey 4 Balmumcu Dispensary, Clinic of Internal Medicine, Istanbul – Turkey 5 Geli?im Medical Laboratory, ?stanbul Yaz??ma Adresi [Correspondence Address] Fatih ?z?elik Laboratory of Clinical Biochemistry Gümu?suyu Military Hospital 34437 Taksim, Istanbul, Turkey Tel: 90-212-2518500 E Mail: 68ozcelik@ Date Registered: 7 January 2010; Date Accepted: 18 August 2010 [Kay?t Tarihi : 7 Ocak 2010; Kabul Tarihi : 18 A?ustos 2010] ABSTRACT Aim: To compare three methods for measurement of blood HbA1c as to reliability, ease and time consumption.” Method: Measurements of HbA1c were made in blood from 120 patients with pre-dia- betes and diabetes using a turbidimetric inhibition immunoassay (TINIA), which also required measurement of total hemoglobin, a particle-enhanced immunoturbidimetric assay (PEITT) without measurement of total hemoglobin, and high performance liquid chromatography (HPLC). Results: There was good concordance between results of PEITT and HPLC methods (r = 0.9401, p0.0001 and by Deming Regression; y = 0.9978x + 0.24). The average HbA1c (7.52±1.40 %) measured by HPLC was higher than the other methods (TINIA: 7.12±1.66 % and PEITT: 7.26±1.39 %, p0.0001). The measured total time spent on 120 samples was 45