Development of an in vitro method to estimate the sensitization induction level of contact allergens.pdfVIP

下载本文档

6
0
约5.18万字
约 11页
2017-03-16 发布于天津
举报
版权申诉

Development of an in vitro method to estimate the sensitization induction level of contact allergens.pdf

1、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Development of an in vitro method to estimate the sensitization induction level of contact allergens.pdf

Toxicology Letters 271 (2017) 1–11 Contents lists available at ScienceDirect Toxicology Letters journal homepage: /locate/toxlet Development of an in vitro method to estimate the sensitization induction level of contact allergens Valentina Galbiatia, Angela Papalea, Marina Marinovicha, Sue Gibbsb,c, Erwin Roggend, Emanuela Corsinia,* a Laboratory of Toxicology, DiSFeB, Università degli Studi di Milano, Milan, Italy b Department of Dermatology, VU University Medical Centre, Amsterdam, The Netherlands c Department of Oral Cell Biology, ACTA, Amsterdam, The Netherlands d 3Rs Management and Consultancy, Denmark HIGHLIGHTS The RhE IL-18 assay better predicts human skin sensitizers. A simple in vitro method to estimate in vivo potency was developed. In the current paper, 14 chemicals not previously tested were used. Good correlations between in vitro predicted LLNA EC3 and human NOEL values with in vivo available data were found. GRAPHICAL ABSTRACT ARTICLE INFO Article history: Received 12 December 2016 Received in revised form 25 January 2017 Accepted 31 January 2017 Available online 9 February 2017 Keywords: Reconstituted human epidermis 3D model In vitro Contact allergens Potency Contact hypersensitivity ABSTRACT No standardized in vitro methods to assess potency of skin sensitizers are available. Recently, we standardized a procedure which combines the epidermal equivalent potency assay with assessment of IL18 to provide a single test for identi?cation and classi?cation of skin sensitizers. This current study aimed to extend tested chemicals, and to provide a simple in vitro method for estimation of the expected sensitization induction level interpolating in vitro EC50 and IL-18 SI2 values to predict LLNA EC3 and/or human NOEL from standards curves generated using reference contact allergens. Reconstituted human epidermis was challenged with 14 chemicals not previously tested benzoquinone, chlorpromazine, chloramine T, benzyl salicylate, diethyl maleate, dihyd