大白菜玻利玛细胞质雄性不育系92―09A的形态特征及相关基因的克隆与表达模式分析.docVIP

大白菜玻利玛细胞质雄性不育系92―09A的形态特征及相关基因的克隆与表达模式分析 　　摘 要：玻利玛细胞质雄性不育（Pol CMS）是目前很有利用价值的胞质雄性不育源。在前期的研究中，我们利用柯桂兰提供的大白菜Pol CMS不育源为母本，以大白菜骨干亲本92-09为轮回亲本，培育出大白菜Pol CMS不育系92-09A。本研究对大白菜Pol CMS不育系92-09A及其保持系92-09、恢复系早28的形态特征进行观察，并对相应的不育基因Braraorf224及其恢复基因BraraPPR进行了克隆及生物信息学分析。结果表明，大白菜Pol CMS不育系92-09A的花瓣、萼片和雌雄蕊都比保持系92-09小，不能形成有活力的成熟花粉粒；其保持系和杂种F1（不育系92-09A×恢复系早28）均可以产生有活力的花粉粒。大白菜Pol CMS不育基因Braraorf224及恢复基因BraraPPR与甘蓝型油菜的Pol CMS不育基因及恢复基因同源性很高。qRT-PCR结果显示，BraraPPR在恢复系早28和杂种F1中的表达量均明显高于雄性不育系92-09A。本研究可为进一步揭示大白菜Pol CMS雄性不育机理奠定基础。 　　关键词：大白菜；Pol CMS；不育基因；恢复基因 　　中图分类号：S634.1+Q78文献标识号：A文章编号：1001-4942（2016）12-0006-10 　　Abstract Polima cytoplasmic male sterile （Pol CMS） is a very important CMS source in plant breeding. In the previous study， we used Pol CMS line KG-Pol of Chinese cabbage provided by Professor Ke Guilan as female parent and core line 92-09 as recurrent parent to breed Pol CMS line 92-09A of Chinese cabbage. Here， the morphological characteristics of Chinese cabbage Pol CMS line 92-09A， its parental maintainer line 92-09 and restorer line Zao 28 were investigated， and the Pol CMS gene Braraorf224 and its restorer gene BraraPPR were cloned and analyzed. The results showed that the petals， sepals， stamens and pistils of Chinese cabbage Pol CMS line 92-09A were all smaller than those of its maintainer line 92-09， and it could not form mature pollen with viability. While the maintainer line 92-09 and the F1 hybrid of Pol CMS line 92-09A×restorer line Zao 28 both had pollen grains with viability. Bioinformatics analysis indicated that the nucleic acids and amino acids of Chinese cabbage Pol CMS gene Braraorf224 and restorer gene BraraPPR were very close to those of Brassica napus.The results of qRT-PCR showed that the expression level of BraraPPR in restorer line Zao 28 and F1 hybrid were both higher than that in Pol CMS line 92-09A. The research would be helpful to understand the molecular mechanism of Pol CMS of Chinese cabbage in the future. 　　Keywords Chinese cabbage （Bras