毛细管气相色谱法同时测定血中醇酮类化合物.doc

全血中乙醇的毛细管气相色谱快速检测法 [中文摘要]：目的 建立全血样品的乙醇直接提取进样-毛细管气相色谱快速分析方法。方法 取全血样品0.50mL，准确加入二甲基亚砜（DMSO）1.00mL和2g无水硫酸钠，振摇、离心后，取上清液1μL直接进样。结果 在选定的色谱条件下，血中的乙醇能与样品中的主要干扰成分实现基线分离。在0.0~300.0mg/100mL范围内线性良好，检出限为0.2mg/100mL。标准溶液测定的相对标准偏差（ ，RSD）为1.36%，将所建立的方法用于全血样品的分析，取得了较为满意的结果，加标回收率为90.9%~107.3%，样品测定的RSD为1.98%。对测定方法的不确定度进行了分析，合成不确定度为2.2%。结论 该法可应用于血中乙醇的测定，样品提取后直接进样分析，方法简单快速，适合大批量样品的快速分析。 [关键词] 毛细管气相色谱，直接进样，乙醇，叔丁醇内标法 Rapid determination of direct sample injection by capillary - gas chromatography Abstract：Objective To establish A rapid determination method of ethanol in blood that the sample is injected directly intowith capillary-GC. Methods After taking 0.50mL of whole blood sample and adding 1.00mL of dimethyl sulfoxide (DMSO), and 2g anhydrous sodium sulfate，the super sample solution was directly injected into GC for analysis. Results The substances of the sample can be separated from ethanol fully under this GC condition. The linear formula of ethanol in blood was Y = 0.0953x－0.0459, r = 0.9993; the liner range for ethanol was 0.0～300.0 mg/100mL and the detection limit was 0.2mg/100mL. The RSD of standard solution determination within - day was 1.36%. The satisfactory results were obtained for whole blood samples with the proposed method. The recoveries were 90.9%~107.3% for ethanol and the RSD for the samples determination was 1.98%. The combined uncertainty was calculated and the value was 2.2%. Conclusion This is a rapid, sensitive and simple method. It can be used for the diagnosis of ethanol in blood. Key words: Capillary – GC; Direct sample injection; Ethanol; Internal standard method 乙醇中毒多发生于饮酒，饮酒后极少量的乙醇在胃内被粘膜内的乙醇脱氢酶代谢清除，而大部分留于小肠内被迅速扩散吸收至体液中。过量饮酒已被列为世界公共卫生的主要问题之一，可诱发多种疾病，如心血管疾病、消化系统疾病、精神障碍等。急性酒精中毒主要表现为神经行为的改变，导致行为异常，从而发生意外甚至犯罪，比如车祸，酒后肇事等[1]。交通事故现已成为社会的第一大公害，给人类带来重大的人员伤亡和财产损失。醉酒驾车是引起交通事故的第一大因素，引起了全社会的重视。饮酒后，大脑由于酒精的作用, 使得各项感官性能下降、注意力分散，从而造成判断和操作的失误，增加交通事故发生的危险性[2]。在交通事故责任认定时，肇事者血液中的乙醇含量是重要的判断依据，因此，如何快速准确的检测血液中乙醇含量，可以为交通事故及时公平的处理提供技术支持和保障。 目前血醇含量的测定多为气相色谱法