分子生物学英文试题..docVIP

Multiple Choice (1) The attachment site for RNA polymerase in bacteria is called the: a. Initiator b. Operator c. Promoter d. Start codon (2) The specificity of bacterial RNA polymerase for their promoters is due to which subunit? a. α b. β c. γ d. σ (3) The first protein complex to bind to the core promoter for a protein-coding gene in eukaryotes is; a. RNA polymerase II b. General transcription factor TFIIB c. General transcription factor TFIID d. General transcription factor TFIIE (4) Which modification must be made to RNA polymerase II in order to activate the preinitiation complex? a. Acetylation b. Methylation c. Phosphorylation d. Ubiquitination (5) What is the name of the DNA sequence that is located near the promoter of the lactose operon, and which regulates expression of the operon in E. coli? a. Activator b. Inducer c. Operator d. Repressor (6) Which of the following types of sequence module enables transcription to respond to general signals from outside of the cell? a. Cell-specific modules b. Developmental modules c. Repression modules d. Response modules (7) Which of the following is NOT a type of activation domain? a. Acidic domains b. Glutamine-rich domains c. Leucine-zipper domains d. Proline-rich domains (8) Which of the following is NOT a experiment used to define the site on a DNA molecule to which a protein binds? a. Gel retardation assay b. DNA footprinting assay c. Modification interference assay d. Yeast two hybrid assay (9) Which of the following DNA sequences can increase the rate of transcription initiation of more than one gene/promoter? a. Activators b. Enhancers c. Silencers d. Terminators (10) Approximately how many base pairs form the attachment between the DNA template and RNA transcript during transcription? a. 8 b. 12-14 c. 30 d. The entire RNA molecule remains base-paired to the template until transcription is finished. (11) Which factor is thought to be most important in determi