离子交换树脂对藤黄果中羟基柠檬酸的分离纯化.docVIP

离子交换树脂对藤黄果中羟基柠檬酸的分离纯化 Separation and purification of ydroxycitric acid from Garcinia cambogia with ion exchange resin 易馨如1,2,3,4 封 棣1,2,3,4 王 泰1,2,3,4 张丞林1,2,3,4 曹学丽1,2,3,4 YI Xin-ru1,2,3,4 FENG Di1,2,3,4 WANG Tai1,2,3,4 ZHANG Cheng-lin1,2,3,4 CAO Xue-li1,2,3,4 (1. 北京工商大学食品学院，北京 100048；2. 食品添加剂与配料北京市高等学校工程中心，北京 100048；3. 北京市食品风味化学重点实验室，北京 100048；4. 食品质量与安全北京实验室，北京 100048） (1. Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing 100048, China; 2. Beijing Laboratory for Food Quality and Safety, School of Food and Chemical Engineering, Beijing 100048, China; 3. Beijing Key Laboratory of Food Flavor Chemistry, Beijing 100048, China; 4. Beijing Technology and Business University, Beijing 100048, China) 摘( 要：对藤黄果中羟基柠檬酸的纯化工艺进行系统的研究，通过对D201、D315、D301、HZ-202和335共5种离子交换树脂对羟基柠檬酸的吸附和解吸能力的比较，筛选出最佳树脂为D201，确定了最佳的吸附与解吸工艺条件，吸附条件：pH=5，上样浓度3.12 mg/mL，上样流速1 mL/min；解吸条件：洗脱液为0.4 mol/L NaCl水溶液，洗脱流速1 mL/min，羟基柠檬酸样品溶液经D201树脂吸附与解吸后纯度由40.3%提高到91.3%。结果表明，D201树脂对羟基柠檬酸的分离纯化具有良好的选择性和应用前景。 关键词：离子交换树脂；藤黄果；羟基柠檬酸；分离；纯化 Abstract：The purification of Hydroxycitric acid from Garcinia cambogia using ion exchange resin was study. Five different kinds of ion exchange resins named D201, D315, D301, HZ-202 and 335 were studied. The static capacity of adsorption and desorption with Hydroxycitric acid was investigated. The resin D201 was selected in the study. The optimum adsorption and desorption parameters for the separation and purification of Hydroxycitric acid were as follows, adsorption: pH 5, sample concentration 3.12 mg/mL, flow rate 1 mL/min; desorption: eluent 0.4 mol/L NaCl aqueous solution, flow rate 1mL/min. After treating with D201, the purity of Hydroxycitric acid increased from 40.3% to 91.3%. The results showed that the resin D201 for the separation and purification of Hydroxycitric acid with good selectivity and application prospect. Keywords: ion exchange resin; Garcinia cambogia; hydroxycitric acid; separation; purification