1菱角粗多糖抗肿瘤活性研究.docVIP

菱角粗多糖抗肿瘤活性研究 牛凤兰1，董卿1，范东艳1，哈卿1 ,孙文怡2 （1.吉林大学公共卫生学院，吉林 长春 130021 2.吉林师范大学 ，吉林 四平136000） 摘 要：目的 对菱角粗多糖进行分离提取及体外抑瘤作用研究。 方法 采用乙醇分级沉淀法分离菱角粗多糖，采用苯酚硫酸法测定其糖含量，并采用噻唑蓝(MTT)法测定对肿瘤细胞增殖的抑制作用，采用流式细胞术分析其中多糖组分F3诱导人脑胶质瘤细胞U251凋亡作用。结果 从菱角中分离得到3个粗多糖组分，菱角粗多糖具有抑制肿瘤细胞增殖及诱导肿瘤细胞调亡的作用，三个组分浓度在7.81、15.63、31.2562.50、125μg/ml范围内对Hela和U251细胞增殖的抑制作用存在明显的量效关系。结论 菱角粗多糖能够抑制Hela和U251两种肿瘤细胞增殖，并诱导其凋亡。 关键词：菱角;多糖;凋亡 Study on the Isolation and Antitumor Activity of Crude Polysaccharides Components from Water-caltrop Niu Fenglan*，Dong Qing，Fan Dongyan, Ha Qing （School of public health，Jilin University，Ji Lin province，Chang Chun，130021） Abstract:Objective：To crude polysaccharides from Water-caltrop and to study their anti-tumor’s effect in vitro. Methods:Ethanol fractionation method was used to separate crude polysaccharides from Water-caltrop. MTT method was used to evaluate the anti-tumor activity in vitro of crude polysaccharides from Water-caltrop. Flow cytometry method was used to detect the apoptosis of U251 cells inducing by the crude polysaccharide F3’s. Results:Three kinds of polysaccharide compounds were obtained by the method of ethanol fractionation from the Water-caltrop. The results of MTT assay showed that the different concentration (7.81、15.63、31.25、62.50 and125.00 μg/ml) of the crude Polysaccharides from Water-caltrop could significantly inhibit the proliferation of Hela and U251 cells cultured in vitro in a dose-dependent manner and reduced the rate of cell apoptosis. The number of two kinds of cells was gradually decreasing with the concentration of the crude polysaccharides from Water-caltrop was increasing. Conclusion:The results proved that crude polysaccharide could inhibit the Hela and U251 cells’ proliferation and induce U251 cell to apoptosis. Keywords: Water-caltrop; polysaccharide;Apoptosis 恶性肿瘤已经成为人类生命的三大杀手之一，医学界在寻求和使用抗癌药物的同时发现许多化学抗癌药物在作用于靶细胞时往往累及正常细胞但植物药的遗传毒性不太明显表明中草药在抗癌抗突变方面有独特的优势和广阔的应用前景。近些年来单味中药及中药复方防治肿瘤作用的研究已取得一定成绩[1] 菱角（Water caltrop）中药大辞典所收载，具有清热、解渴、健脾、益气明目之功效[]。前