论文-华西蟾蜍.docVIP

华西蟾蜍睾丸的组织学观察 郑朝燕 生命科学学院生物科学专业2008级2班 指导教师：米志平 摘 要:本文对华西蟾蜍的睾丸组织结构进行了研究。结果表明:在光学显微镜下观察，性成熟的华西蟾蜍睾丸表面被以浆膜，其下为致密结缔组织，内部由曲细精管及其间的疏松结缔组织，血管，黑色素细胞和间质细胞组成。曲细精管由基膜、包括生精细胞和支持细胞的生精上皮组成。在曲细精管横切面，可看见由外向管腔内，精原细胞紧贴基膜，初级精母细胞、次级精母细胞、精细胞、精子、依次向管腔推进，最后将成熟的精释放到曲细精管管腔。并且在减数分裂形成精子的各个时期，处于相同时期的细胞成团存在，称之为精子发生囊。 关键词:睾丸，组织学，华西蟾蜍。 Histological observation of the testes of Bufo andrewsi ZHENG Chao-yanClass 2, Grade 2008, College of Life Science Instructor: MI Zhi-ping Abstract: In this paper, the histo-structure of the testis of Bufo andrewsi was studied studied .The results showed that: under the optical microscope, Bufo andrewsis testis was covered by serosa, under the serosa, there was dense connective tissue, the substance of the testis was composed of seminiferous tubules and loose connective tissue, blood vessels, melanoma cells and interstitial cells. The seminiferous tubule had an outer layer of basal cells, basement membrane and reproductive epithelium. Spermatogenic epithelium was composed of spermatogenic cells and Sertoli cells. In the cross section of seminiferous tubules, from outside to the inside lumen, spermatogonial cells close to the basal lamina, primary spermatocytes, secondary spermatocytes, spermatids, sperm advance to the lumen in proper sequence, finally release the mature sperm into the lumen. And in each period of the meiosis to produce sperm, the cell of the same period are gathered the group which called spermatogenesis cyst. Key words: Testes; Histology; Bufo andrewsi 蟾蜍在医学和生物学试验上应用较多，同时是重要的动物药物资源,“蟾酥，蟾衣，都是极其珍贵的中药材Bufo andrewsi)是隶属于脊索动物门 Chordata) 脊椎动物亚门 Vertebrata) 两栖纲 Amphibia) 无尾目 Salientia)新蛙亚目 Neobatrachia ) 蟾蜍科 Bufonidae ) 蟾蜍属Bufo )。在我国广泛于四川、云南、贵州、陕西、甘肃、湖北、广西及广东等地[1]。 华西蟾蜍很少主动地攻击和主动地防御，常常成为蛇类和鸟类的口中餐，为了保护自己，华西蟾蜍发展了一种被动的防御方式：通过体色来保护自己。它们的体色以乳黄色为主，与枯树叶、泥土的颜色非常相似，一旦与环境色融为一体，蛇和鸟就很难发现它们了。当然，体色仅是华西蟾蜍保护自己的一种方式，它们有毒的腺体也可以帮助它们防御敌害侵袭。华西蟾蜍穴居在泥土中，或栖于石下及草间；栖居草丛、石下或土洞中，黄昏爬出捕食。成蟾在水底泥土或烂草中冬眠。其蝌蚪喜成群朝同一方向游动。冬季多在水底泥中。白昼潜伏，晚上或雨天外出活动。以捕获蜗牛、蛞蝓、蚂蚁、甲虫与蛾类等