Intravasculartraffickingofdual-colorHT-1080humanfibros.pptVIP

Intravascular trafficking of dual-color HT-1080 human fibrosarcoma cells labeled in the nucleus with histone H2B-GFP and in the cytoplasm with RFP. A, schematic diagram of the skin flap model in live mice for imaging intravascular trafficking and extravasat... Kensuke Yamauchi et al. Cancer Res 2006;66:4208-4214 ?2006 by American Association for Cancer Research Intravascular trafficking of dual-color HT-1080 human fibrosarcoma cells labeled in the nucleus with histone H2B-GFP and in the cytoplasm with RFP. A, schematic diagram of the skin flap model in live mice for imaging intravascular trafficking and extravasation. An arc-shaped incision was made in the abdominal skin, and then the skin flap was spread and fixed on a flat stand. Dual-color HT-1080 cells are injected into the epigastrica cranialis vein through a catheter. Immediately after injection, the inside surface of the skin flap was directly observed. B, dual-color HT-1080 cell crawls smoothly along the vessel wall without rolling in a capillary arrow . The nucleus and cytoplasm are slightly stretched. The nucleus is in the front of the cell while the cell is crawling. When the cell advances into a part of the capillary where the diameter is smaller than of deformation limit of the cell, the cell does not advance any further. Bar, 100 μm. C, dual-color HT-1080 cell, trafficking at low velocity, advances between other cells and the vessel wall. The moving cancer cell contacts the other cells arrow . The cell deforms slightly and continues to move without adhesion. Bar, 100 μm. Right, schematics of B and C . D, One cancer cell migrating in the post capillary with slow velocity. The cytoplasm is at the head of the cell while the cell is moving in a large vein, but the nucleus is at the head in a small vein. The velocity of the cells in A and B is an average of 24.2 μm/s. The average velocity in cells in D and E , however, is only 6.1 μm/s because the cells are in a narrower vein. Images were taken