生物大分子相互作用医学分子生物学.pptVIP

* Others such as lipids, * Others such as lipids, * * SPR: advantages applications 优点： 无需对样品进行标记 实时监测 灵敏度高 快速 用处： 特异性—哪些分子发生了相互作用? 浓度—存在多少结合分子? 动力学—相互作用的速率、结合和解离的比例是多少? 亲和性—相互作用的程度有多大? 协同作用—是否存在任何异构效应? 相互作用模式—结合模式与不同样品是否存在对应关系？ SPR: applications Drug screening Kinetics studies Cell signaling Multi-molecular complexes Enzyme reaction Ligand-receptor reaction Vaccine development Cancer reasearch Proteomics …… SPR: further development 生物分子相互作用分析-质谱(BIA-MAS): 是SPR-BIA技术与传统的蛋白鉴定技术MALDI-TOF 有机结合而形成的一种新的研究手段。 TRENDS in Biotechnology Vol.21 No.7 July 2003 Protein-protein Interaction Databases BIND (Biomolecular Interaction Network Database) DIP (Database of Interacting Proteins) IntAct HPRD (Human Protein Reference Database) MINT (Molecular INTeraction database) MIPS (Mammalian Protein–Protein Interaction Database) Protein-protein Interaction Databases 名称 地址 蛋白质数量* PPI数量* 数据来源 数据存储 格式 是否提供可视化网络分析 特性 BIND http://bind.ca 未知 200,000 文献挖掘 高通量 XML,ASN.1, BioPAX,CSV,SIF,XML 否 Ontoglyph DIP / 19,344 63,168 文献挖掘 MIF,XIN 是 PPI真实性评估 IntAct http://www.ebi.ac.uk/intact/site 56,071 138,931 文献挖掘 PSI-MI 是 控制词汇，预测pull-down实验 HPRD / 25,205 37,581 文献挖掘 PSI-MI 否 PhosphoMotif Finder MINT http://mint.bio.uniroma2.it/mint/ 28,186 103,808 文献挖掘 PSI-MI, Osprey 是 HomoMINT MIPS http://mips.gsf.de/ 900 1,800 文献挖掘 PSI-MI,XML 否 PEDANT The usage of PPI resources Protein-protein interactions: binary interactions, molecular complexes The network of interacting proteins Pathways (functional) Evolution of protein-protein interaction The possible interaction network of angiogenin and its partners Protein-DNA interaction The interaction between protein and DNA happens in the nucleus transcription regulation: transcription factors replication repair epigenetic regulation focus: the interaction between cis-acting factor and trans-acting element The methods commonly used for studying protein-DNA interaction Nuclease