Molecular mechanism of serinethreonine protein phosphatase 1 (PP1cα–PP1r7) in spermatogenesis of Toxocara canis》.pdfVIP

Acta Tropica 149 (2015) 148–154 Contents lists available at ScienceDirect Acta Tropica j o u rn al h omepage: w w w. elsev /locate/actatropica Molecular mechanism of serine/threonine protein phosphatase 1 (PP1c–PP1r7) in spermatogenesis of Toxocara canis Guang Xu Ma a , Rong Qiong Zhou a,∗ , Zhen Hui Song a , Hong Hong Zhu a , Zuo Yong Zhou a , Yuan Qin Zeng b a Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, People’s Republic of China b College of Life Sciences, Southwest University, Chongqing 402460, People’s Republic of China a r t i c l e i n f o a b s t r a c t Article history: Toxocariasis is one of the most important, but neglected, zoonoses, which is mainly caused by Toxocara Received 21 March 2015 canis. To better understand the role of serine/threonine protein phosphatase 1 (PP1) in reproductive Received in revised form 20 May 2015 processes of male adult T. canis, differential expression analysis was used to reveal the profiles of PP1 Accepted 25 May 2015 catalytic subunit (PP1c) gene Tc-stp-1 and PP1 regulatory subunit 7 (PP1r7) gene TcM-1309. Indirect Available online 27 May 2015 fluorescence immunocytochemistry was carried out to determine the subcellular distribution of PP1c. Double-stranded RNA interference (RNAi) assays were employed to illustrate the function and mech- Keywords: anism o