Molecular regulation of catechins biosynthesis in tea [Camellia sinensis (L.) O. Kuntze]》.pdfVIP

Gene 495 (2012) 205–210 Contents lists available at SciVerse ScienceDirect Gene jour nal homepage: /locate/gene Short Communication Molecular regulation of catechins biosynthesis in tea [Camellia sinensis (L.) O. Kuntze] Arti Rani 1,2, Kashmir Singh 2,3, Paramvir S. Ahuja, Sanjay Kumar ⁎ Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology (Council of Scientifi c and Industrial Research, CSIR), P.O. Box No. 6, Palampur-176061, India a r t i c l e i n f o a b s t r a c t Article history: Catechins are bioprospecting molecules present in tea and any effort towards metabolic engineering of this Accepted 15 December 2011 important moiety would require knowledge on gene regulation. These are synthesized through the activities Available online 29 December 2011 of phenylpropanoid and flavonoid pathways. Expression regulation of various genes of these pathways namely phenylalanine ammonia-lyase (CsPAL), cinnamate 4-hydroxylase (CsC4H), p-coumarate:CoA ligase (Cs4CL),fl ava- Keywords: none 3-hydroxylase (CsF3H), dihydrofl avonol 4-reductase (CsDFR) and anthocyanidin reductase (CsANR) was Anthocyanidin synthase Chalcone isomerase accomplished previously. In depth analyses of the remaining genes namely, chalcone synthase (CsCHS), chalcone Flavonoid isomerase (CsCHI),fl avonoid 3′5′-hydroxylase (CsF3′5′H